IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation

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==Ligation strategy==
==Ligation strategy==
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the prey]
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the predator]
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==Quality control==
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Quality control for the prey part]
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<u><font size="2">'''Ligating the Prey Construct:'''</font></u>
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Quality control for the predator part]
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__NOTOC__
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{| border="1" cellpadding="0" width="100%"
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|-
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!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37034</bbpart>=<br>(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:C0261 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]</center>
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!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center>
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|}
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<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
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__NOTOC__
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{| border="1" cellpadding="0" width="100%"
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|-
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!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4
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!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || ||  ||
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!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:J37023 logo.png]]</center> || ||
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!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)</center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:J37024 logo.png]]</center> ||
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!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || ||
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!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center> || ||
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!style="background:#D9F2ED"|<center><bbpart>J37036</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)</center>|| ||  || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center>
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|}
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<u><font size="2">'''Ligating the Monocystronic Predator Construct:'''</font></u>
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__NOTOC__
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{| border="1" cellpadding="0" width="100%"
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!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4 !! style="background:#E6B3E0" |Stage 5
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!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]]</center> || || || ||
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!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:J37024 logo.png|70px]]</center> || || ||
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!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)</center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> || ||
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!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png|30px]]</center> || || || ||
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!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]]</center> || || ||
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!style="background:#D9F2ED"|<center><bbpart>J37031</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" |[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0014 logo.png|50px]]
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!style="background:#D9F2ED"|<center><bbpart>J37018</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)</center>||  ||  || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:Plus sign.png|20px]][[Image:R0062 logo.png|30px]]</center>
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!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
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|}
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==Quality control==
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__NOTOC__
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{| border="1" cellpadding="0" width="100%"
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!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
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| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
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Sequence: TAT TGT CTC ATG AGC GG<br>
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Seconday Structure: None
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| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
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| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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|}
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==WetLab work entries==
==WetLab work entries==

Revision as of 23:17, 29 October 2006

Super Parts Not applicable
Actual Part Image:J37036 part.png
Sub Parts Prey Molecule Generator Predator Molecule Generator


Ligation strategy

Quality control

WetLab work entries

  • The prey has been constructed and tested but is waiting parameter extraction.
  • The predator construct has not been ligated.
  • Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
  • We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.


  • FV:To finish page:
  • Need sequencing results for J37023 and any intermediates
  • Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.
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