IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation

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(Ligation strategy)
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the prey]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation#Quality_control Ligations for the prey]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the predator]
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Implementation Ligations for the predator]
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*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].
==Quality control==
==Quality control==

Revision as of 22:33, 29 October 2006

Super Parts Not applicable
Actual Part Image:J37036 part.png
Sub Parts Prey Molecule Generator Predator Molecule Generator


Ligation strategy

  • More information on ligations can be found on the Ligation Page.

Quality control

Implementation

  • The oscillator will be tested in a chemostat. This equipment works to:
  • Keep the cells in the exponential growth phase
  • Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
  • Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat

WetLab work entries

  • The prey has been constructed and tested but is waiting parameter extraction.
  • The predator construct has not been ligated.
  • Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
  • We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.


  • FV:To finish page:
  • Need sequencing results for J37023 and any intermediates
  • Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.
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