IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation: Difference between revisions
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==Motivations== | ==Motivations== | ||
*To see whether oscillations are produced by measuring fluctuating levels of AHL | *To see whether oscillations are produced by measuring fluctuating levels of AHL that match our specifications: | ||
* | ::*Stable oscillations for greater than 10 cycles | ||
::*High signal to noise ratio | |||
::*Control over frequency and amplitude | |||
==Protocol== | ==Protocol== | ||
Revision as of 09:35, 28 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Construct | Predator Construct |
Enviroment
This system will run in a Chemostat. This pice of equipment will keep the cell populations constant and thus keep the pareameters constant throught the course of the experiment. chemostat page
Motivations
- To see whether oscillations are produced by measuring fluctuating levels of AHL that match our specifications:
- Stable oscillations for greater than 10 cycles
- High signal to noise ratio
- Control over frequency and amplitude
Protocol
- A formal protocol has not been written.
- A suggested method was to aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay
Results
- the construct is not yet available
