IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation: Difference between revisions
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!height="25pt" width="80pt"|Actual Part | !height="25pt" width="80pt"|Actual Part | ||
!colspan="2"| | !colspan="2"| Full System Logo | ||
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!height="25pt" width="80pt"|Sub Parts | !height="25pt" width="80pt"|Sub Parts | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey_Construct| Prey | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey_Construct| Prey Molecule Generator]] | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator_Construct| Predator | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator_Construct| Predator Molecule Generator]] | ||
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<div class="tabs-blue"> | <div class="tabs-blue"> | ||
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::*Stable oscillations for greater than 10 cycles | ::*Stable oscillations for greater than 10 cycles | ||
::*High signal to noise ratio | ::*High signal to noise ratio | ||
::*Controllable frequency | ::*Controllable amplitude and frequency | ||
::*(Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification). | |||
==Protocol== | ==Protocol== | ||
*A formal protocol has not been written. | *A formal protocol has not been written. | ||
* | *Methods for testing under consideration are: | ||
::*To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay. | |||
::*Use a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface biosensor] to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium). | |||
::*Use High Performance Liquid Chromatography (more research is required). | |||
==Results== | ==Results== | ||
*The predator construct | |||
*The prey construct has been built but still needs to be characterized | |||
*The predator construct still needs to be built | |||
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Latest revision as of 09:40, 30 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | Full System Logo | |
| Sub Parts | Prey Molecule Generator | Predator Molecule Generator |
Motivations
- To see whether oscillations, which match are specifications, are produced. We will have fulfilled our specifications if the oscillations have the following properties:
- Stable oscillations for greater than 10 cycles
- High signal to noise ratio
- Controllable amplitude and frequency
- (Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).
Protocol
- A formal protocol has not been written.
- Methods for testing under consideration are:
- To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay.
- Use a biosensor to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium).
- Use High Performance Liquid Chromatography (more research is required).
Results
- The prey construct has been built but still needs to be characterized
- The predator construct still needs to be built
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