IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
|||
| Line 42: | Line 42: | ||
*The prey construct has been built but still needs to be characterized | *The prey construct has been built but still needs to be characterized | ||
*The predator construct still needs to be built | *The predator construct still needs to be built | ||
<html> | |||
<!-- Start of StatCounter Code --> | |||
<script type="text/javascript" language="javascript"> | |||
var sc_project=1999441; | |||
var sc_invisible=1; | |||
var sc_partition=18; | |||
var sc_security="18996820"; | |||
</script> | |||
<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&java=0&security=18996820&invisible=1" alt="website statistics" border="0"></a> </noscript> | |||
<!-- End of StatCounter Code --> | |||
</html> | |||
Latest revision as of 09:40, 30 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | Full System Logo | |
| Sub Parts | Prey Molecule Generator | Predator Molecule Generator |
Motivations
- To see whether oscillations, which match are specifications, are produced. We will have fulfilled our specifications if the oscillations have the following properties:
- Stable oscillations for greater than 10 cycles
- High signal to noise ratio
- Controllable amplitude and frequency
- (Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).
Protocol
- A formal protocol has not been written.
- Methods for testing under consideration are:
- To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay.
- Use a biosensor to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium).
- Use High Performance Liquid Chromatography (more research is required).
Results
- The prey construct has been built but still needs to be characterized
- The predator construct still needs to be built
<html> <!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=1999441; var sc_invisible=1; var sc_partition=18; var sc_security="18996820"; </script>
<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&java=0&security=18996820&invisible=1" alt="website statistics" border="0"></a> </noscript> <!-- End of StatCounter Code --> </html>
