IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
(Motivations)
Line 29: Line 29:
::*Controllable amplitude and frequency
::*Controllable amplitude and frequency
::*(Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).
::*(Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).
-
*The testing will be done in a [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation/chemostat|chemostat]]. This equipment works to:
 
-
::*Keep the cells in the exponential growth phase
 
-
::*Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
 
-
::*Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat
 
==Protocol==
==Protocol==

Revision as of 23:21, 29 October 2006

Super Parts Not applicable
Actual Part Full System Logo
Sub Parts Prey Molecule Generator Predator Molecule Generator


Motivations

  • To see whether oscillations, which match are specifications, are produced. We will have fulfilled our specifications if the oscillations have the following properties:
  • Stable oscillations for greater than 10 cycles
  • High signal to noise ratio
  • Controllable amplitude and frequency
  • (Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).

Protocol

  • A formal protocol has not been written.
  • Methods for testing under consideration are:
  • To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay.
  • Use a biosensor to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium).
  • Use High Performance Liquid Chromatography (more research is required).

Results

  • The prey construct has been built but still needs to be characterized
  • The predator construct still needs to be built
Personal tools