IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation

Super Parts	Not applicable
Actual Part
Sub Parts	Prey Construct	Predator Construct

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Motivations

• To see whether oscillations are produced by measuring fluctuating levels of AHL that match our specifications:

• Stable oscillations for greater than 10 cycles
• High signal to noise ratio
• Control over frequency and amplitude

Enviroment

This system will run in a Chemostat. This pice of equipment will keep the cell populations constant and thus keep the pareameters constant throught the course of the experiment. chemostat page

Protocol

• A formal protocol has not been written.
• A suggested method was to aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay

Results

• The predator construct has to be built.