IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation: Difference between revisions
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*The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information] | *The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information] | ||
*Reasons for parts not being built include time constraint (and perhaps non-functional registry parts). | *Reasons for parts not being built include time constraint (and perhaps non-functional registry parts). | ||
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Latest revision as of 09:43, 30 October 2006
Super Parts | Molecular Prey-Predator Oscillator | |
---|---|---|
Actual Part | ||
Sub Parts | Test Sensing Predator Construct | Test Killing Predator Construct |
Ligation strategy
Ligating the Polycystronic Predator Construct:
Ligating the Monocystronic Predator Construct:
- More information on ligations can be found on the Ligation Page.
Quality control
Part | Logo | Built | Sequenced | Primers Used for Sequencing |
---|---|---|---|---|
No | N/A | N/A | ||
Yes | Yes | Primers used | ||
Yes | Yes Note: This part was sequenced as a part of J37025 |
Primers used | ||
No | N/A | N/A | ||
Yes | Yes | Primers used | ||
Yes | No | N/A | ||
Yes | No | N/A | ||
No | N/A | N/A | ||
No | N/A | N/A |
WetLab work entries
- the final assembly was not successful
- The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
- Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).
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