IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation

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(Quality control)
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==Ligation strategy==
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<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
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*For predator sensing characterisation, we planned to build :
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__NOTOC__
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**[http://parts.mit.edu/registry/index.php/Part:BBa_J37016 J37016]
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{| border="1" cellpadding="0" width="100%"
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**[http://parts.mit.edu/registry/index.php/Part:BBa_J37020 J37020]
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|-
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*For predator killing characterisation, we planned to build :
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!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4
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**[http://parts.mit.edu/registry/index.php/Part:BBa_J37023 J37023]
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|-
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**[http://parts.mit.edu/registry/index.php/Part:BBa_J37024 J37024]
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!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || ||  ||
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*For overall predator construct, we planned to build
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|-
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**[http://parts.mit.edu/registry/index.php/Part:BBa_J37035 J37035]
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!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:J37023 logo.png]]</center> || ||
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**[http://parts.mit.edu/registry/index.php/Part:BBa_J37036 J37036]
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)</center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:J37024 logo.png]]</center> ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center> || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37036</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)</center>|| ||  || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center>
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|}
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==Quality control==
 
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*define the quality control used at each step
 
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*define possible enzymes to be used and their outcomes
 
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*define design of primers to be used in sequencing
 
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 +
<u><font size="2">'''Ligating the Monocystronic Predator Construct:'''</font></u>
__NOTOC__
__NOTOC__
{| border="1" cellpadding="0" width="100%"
{| border="1" cellpadding="0" width="100%"
|-
|-
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!height="25pt" width="80pt"| Part !! Built !! Sequencing Results !! Primers Used for Sequencing
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!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4 !! style="background:#E6B3E0" |Stage 5
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]]</center> || || || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:J37024 logo.png|70px]]</center> || || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)</center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png|30px]]</center> || || || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]]</center> || || ||
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37031</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" |[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0014 logo.png|50px]]
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37018</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)</center>||  ||  || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:Plus sign.png|20px]][[Image:R0062 logo.png|30px]]</center>
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
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|}
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==Quality control==
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__NOTOC__
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{| border="1" cellpadding="0" width="100%"
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|-
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!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
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|-
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| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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|-
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| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
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Sequence: TAT TGT CTC ATG AGC GG<br>
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Seconday Structure: None
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|-
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| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
|-
|-
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|Put part logo and name here || has it been built? || sequencing analysis and link to sequence results || primers used
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| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|-
|-
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|Put part logo and name here || has it been built? || sequencing analysis and link to sequence results || primers used
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| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|-
|-
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| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|}
|}

Revision as of 23:02, 29 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Predator Construct Test Killing Predator Construct


Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
Image:J37023 logo.png
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
Image:B0034 logo.pngImage:J37023 logo.png
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
Image:B0034 logo.pngImage:J37024 logo.png
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37036 logo.png


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
Image:J37016 logo.png Yes ? ?
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Forward pSB1(AK3/A3/A2)

Sequence: TAT TGT CTC ATG AGC GG
Seconday Structure: None

<bbpart>J37020</bbpart>
Image:J37020 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes ? ?
<bbpart>J37032</bbpart>
Image:J37032 logo.png Yes ? ?
<bbpart>J37033</bbpart>
Image:J37033 part.png Yes ? ?

WetLab work entries

  • the final assembly was not successful
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