IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation

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==Ligation strategy==
<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
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!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
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*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].
==Quality control==
==Quality control==

Revision as of 23:30, 29 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Predator Construct Test Killing Predator Construct


Ligation strategy

Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
Image:J37023 logo.png
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
Image:B0034 logo.pngImage:J37023 logo.png
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
Image:B0034 logo.pngImage:J37024 logo.png
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37036 logo.png


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)
  • More information on ligations can be found on the Ligation Page.

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37018</bbpart>
Image:J37018 logo.png No N/A N/A
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Forward pSB1(AK3/A3/A2)

Sequence: TAT TGT CTC ATG AGC GG
Seconday Structure: None

<bbpart>J37023</bbpart>
Image:J37023 logo.png Yes ? ?
<bbpart>J37024</bbpart>
Image:J37024 logo.png No N/A N/A
<bbpart>J37025</bbpart>
Image:J37025 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png ? ? ?
<bbpart>J37033</bbpart>
Image:J37033 logo.png Yes ? ?
<bbpart>J37035</bbpart>
Image:J37035 logo.png No N/A N/A
<bbpart>J37036</bbpart>
Image:J37036 part.png No N/A N/A

WetLab work entries

  • the final assembly was not successful
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