IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation

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(Quality control)
(WetLab work entries)
Line 99: Line 99:
*the final assembly was not successful
*the final assembly was not successful
 +
*The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information]
 +
*Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).

Revision as of 10:15, 30 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Predator Construct Test Killing Predator Construct


Ligation strategy

Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
Image:J37023 logo.png
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
Image:B0034 logo.pngImage:J37023 logo.png
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
Image:B0034 logo.pngImage:J37024 logo.png
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37036 logo.png


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)
  • More information on ligations can be found on the Ligation Page.

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37018</bbpart>
Image:J37018 logo.png No N/A N/A
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Primers used
<bbpart>J37023</bbpart>
Image:J37023 logo.png Yes Yes
Note: This part was sequenced as a part of J37025
Primers used
<bbpart>J37024</bbpart>
Image:J37024 logo.png No N/A N/A
<bbpart>J37025</bbpart>
Image:J37025 logo.png Yes Yes Primers used
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes No N/A
<bbpart>J37033</bbpart>
Image:J37033 logo.png Yes No N/A
<bbpart>J37035</bbpart>
Image:J37035 logo.png No N/A N/A
<bbpart>J37036</bbpart>
Image:J37036 part.png No N/A N/A

WetLab work entries

  • the final assembly was not successful
  • The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
  • Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).
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