IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Design: Difference between revisions
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!height="25pt" width="80pt"| Super Parts | !height="25pt" width="80pt"| Super Parts | ||
!colspan=" | !colspan="3" | [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full_System | Molecular Prey-Predator Oscillator]] | ||
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!height="25pt" width="80pt"|Actual Part | !height="25pt" width="80pt"|Actual Part | ||
!colspan=" | !colspan="3"| [[Image:J37015page.JPG]] | ||
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!height="25pt" width="80pt"|Sub Parts | !height="25pt" width="80pt"|Sub Parts | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test_Sensing_Prey_Construct| Test Sensing Prey Construct]] | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test_Sensing_Prey_Construct| Test Sensing Prey Construct]] | ||
! | ! <bbpart>C0261</bbpart> | ||
! <bbpart>I13401</bbpart> | |||
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<div class="tabs-blue"> | <div class="tabs-blue"> | ||
Revision as of 16:11, 29 October 2006
| Super Parts | Molecular Prey-Predator Oscillator | ||
|---|---|---|---|
| Actual Part | 
| ||
| Sub Parts | Test Sensing Prey Construct | <bbpart>C0261</bbpart> | <bbpart>I13401</bbpart> |
Registry
J37015
Design Choices
- Design allows for an exponential increase of AHL via a positive feedback loop
- Interaction between LuxR, AHL and promoter controlling expression of LuxI
- Design allows for assesment of status of positive feedback loop via:
- GFP Production - Linked to LuxI expression
- AHL Production - Can be indirectly assessed via T9002 assay
| INPUTS | biological component | comments |
|---|---|---|
| AHL | LuxR + pLuxR | These components sense input and activate output |
| OUTPUTS | biological component | comments |
| AHL | LuxI | LuxI expression causes enzymatic generation of AHL |
