IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Design

Super Parts	Molecular Prey-Predator Oscillator
Actual Part
Sub Parts	Test Sensing Prey Construct	<bbpart>C0261</bbpart>	<bbpart>I13401</bbpart>

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Registry

J37015

J37015 @ MIT Parts Registry

Design Choices

• Design allows for an exponential increase of AHL via a positive feedback loop
  • Interaction between LuxR, AHL and promoter controlling expression of LuxI
• Design allows for assesment of status of positive feedback loop via:
  • GFP Production - Linked to LuxI expression
  • AHL Production - Can be indirectly assessed via T9002 assay

INPUTS	biological component	comments
AHL	LuxR + pLuxR	These components sense input and activate output
OUTPUTS	biological component	comments
AHL	LuxI	LuxI expression causes enzymatic generation of AHL