IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation: Difference between revisions

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|-
|-
!height="25pt" width="80pt"| Super Parts
!height="25pt" width="80pt"| Super Parts
!colspan="2" | [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full_System | Molecular Prey-Predator Oscillator]]
!colspan="3" | [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full_System | Molecular Prey-Predator Oscillator]]
|-
|-
!height="25pt" width="80pt"|Actual Part
!height="25pt" width="80pt"|Actual Part
!colspan="2"| Logo of the Part
!colspan="3"| [[Image:J37015page.JPG]]
|-
|-
!height="25pt" width="80pt"|Sub Parts
!height="25pt" width="80pt"|Sub Parts
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test_Sensing_Prey_Construct| Test Sensing Prey Construct]]  
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test_Sensing_Prey_Construct| Test Sensing Prey Construct]]  
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test_Prey_Construct| Test Prey Construct]]
! <bbpart>C0261</bbpart>
! <bbpart>I13401</bbpart>
|}
|}
<div class="tabs-blue">
<div class="tabs-blue">
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<br style="clear:both">
<br style="clear:both">


*Implementation
==Ligation Strategy==
*Implementation
 
*Implementation
<u><font size="2">'''Ligating the Prey Construct:'''</font></u>
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2
|-
!style="background:#D9F2ED"|<center><bbpart>J37034</bbpart>=<br>(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:C0261 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center>
|}
 
 
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].
 
==Quality control==
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
|-
| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || [http://openwetware.org/images/9/9f/Sequence_alignment_22nd_sep.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || [http://openwetware.org/images/b/b1/J37015_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|}
 
==WetLab work entries==
 
*Part has been successfully built.
*Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.
 
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Latest revision as of 09:42, 30 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Prey Construct <bbpart>C0261</bbpart> <bbpart>I13401</bbpart>


Ligation Strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37034</bbpart>
 Yes Yes Primers used
<bbpart>J37015</bbpart>
 Yes Yes Primers used

WetLab work entries

  • Part has been successfully built.
  • Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.

<html> <!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=1999441; var sc_invisible=1; var sc_partition=18; var sc_security="18996820"; </script>

<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&amp;java=0&amp;security=18996820&amp;invisible=1" alt="website statistics" border="0"></a> </noscript> <!-- End of StatCounter Code --> </html>

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