IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation: Difference between revisions
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*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page]. | |||
==Quality control== | ==Quality control== | ||
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!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing | !height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing | ||
|- | |- | ||
| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || | | <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || [http://openwetware.org/images/9/9f/Sequence_alignment_22nd_sep.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used] | ||
|- | |- | ||
| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || | | <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || [http://openwetware.org/images/b/b1/J37015_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used] | ||
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*Part has been successfully built. | *Part has been successfully built. | ||
*Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested. | *Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested. | ||
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Latest revision as of 09:42, 30 October 2006
| Super Parts | Molecular Prey-Predator Oscillator | ||
|---|---|---|---|
| Actual Part | 
| ||
| Sub Parts | Test Sensing Prey Construct | <bbpart>C0261</bbpart> | <bbpart>I13401</bbpart> |
Ligation Strategy
Ligating the Prey Construct:
| For | Stage 1 | Stage 2 |
|---|---|---|
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>) |
   | |
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>) |
 |
- More information on ligations can be found on the Ligation Page.
Quality control
| Part | Logo | Built | Sequenced | Primers Used for Sequencing |
|---|---|---|---|---|
 |
Yes | Yes | Primers used | |
 |
Yes | Yes | Primers used |
WetLab work entries
- Part has been successfully built.
- Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.
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