IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation

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==Ligation Strategy==
==Ligation Strategy==
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*<bbpart>F2620</bbpart> (6B) + <bbpart>J37034</bbpart>
+
<u><font size="2">'''Ligating the Prey Construct:'''</font></u>
-
**J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp
+
__NOTOC__
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**F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp
+
{| border="1" cellpadding="0" width="100%"
-
*Miniprep sizes expected: 2613 bp & 2079 bp (pSB1A2)
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|-
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*Status: Completed & Sequenced
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!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37034</bbpart>=<br>(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:C0261 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]</center>
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|-
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!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center>
 +
|}
 +
 
 +
 
 +
==Quality control==
 +
__NOTOC__
 +
{| border="1" cellpadding="0" width="100%"
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|-
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!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
 +
|-
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| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
 +
|-
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| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
 +
|}
 +
 
 +
==WetLab work entries==
 +
 
 +
*Part has been successfully built.
 +
*Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.

Revision as of 22:21, 29 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part Image:J37015page.JPG
Sub Parts Test Sensing Prey Construct <bbpart>C0261</bbpart> <bbpart>I13401</bbpart>


Ligation Strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
Image:C0261 logo.pngImage:I13504 logo.png
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)
Image:J37015 logo.png


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37034</bbpart>
 Image:J37034 logo.png Yes ? ?
<bbpart>J37015</bbpart>
 Image:J37015 part.png Yes ? ?

WetLab work entries

  • Part has been successfully built.
  • Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.
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