IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Killing Predator Construct/Implementation: Difference between revisions

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|-
!height="25pt" width="80pt"|Actual Part
!height="25pt" width="80pt"|Actual Part
!colspan="4"| Logo of the Part
!colspan="4"| AiiA Testing Construct <bbpart>J37022</bbpart>[[Image:J37022a.png]]
|-
|-
!height="25pt" width="80pt"|Sub Parts
!height="25pt" width="80pt"|Sub Parts
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]
! <bbpart>R0010</bbpart>
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]
! <bbpart>B0034</bbpart>
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]
! <bbpart>J37023</bbpart>
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]
! <bbpart>B0015</bbpart>
|}
|}
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==Ligation Strategy==


*Implementation
<u><font size="2">'''Ligating the AiiA Testing Construct:'''</font></u>
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3
 
|-
!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart> <br> (PCR'd)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:Plus sign.png|20px]][[Image:B0014 logo.png]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37022</bbpart>=<br>(<bbpart>J04500</bbpart>+<bbpart>J37024</bbpart>)</center>|| || !!style="background:#CCEBAD" | <center>[[Image:J37022 logo.png]]</center>
|}
 
 
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].
 
==Quality control==
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
|-
| <center><bbpart>J37022</bbpart></center> || [[Image:J37022 logo.png]] || No || N/A || N/A
|-
| <center><bbpart>J37023</bbpart></center> || [[Image:J37023 logo.png]] || Yes || [http://openwetware.org/images/b/b3/J37025sequencing.doc Yes] <br> Note: This part was sequenced as a part of J37025 || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
| <center><bbpart>J37024</bbpart></center> || [[Image:J37024 logo.png]] || No || N/A || N/A
|}
 
==WetLab work entries==
 
*The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information]
*Parts J37024 and J37022 were not built due to time constraints.

Latest revision as of 08:06, 30 October 2006

Super Parts Predator Construct
Actual Part AiiA Testing Construct <bbpart>J37022</bbpart>
Sub Parts <bbpart>R0010</bbpart> <bbpart>B0034</bbpart> <bbpart>J37023</bbpart> <bbpart>B0015</bbpart>


Ligation Strategy

Ligating the AiiA Testing Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37023</bbpart>
(PCR'd)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37022</bbpart>=
(<bbpart>J04500</bbpart>+<bbpart>J37024</bbpart>)


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37022</bbpart>
 No N/A N/A
<bbpart>J37023</bbpart>
 Yes Yes
Note: This part was sequenced as a part of J37025		 Primers used
<bbpart>J37024</bbpart>
 No N/A N/A

WetLab work entries

  • The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
  • Parts J37024 and J37022 were not built due to time constraints.
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