IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Killing Predator Construct/Implementation: Difference between revisions
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==Ligation | ==Ligation Strategy== | ||
<u><font size="2">'''Ligating the AiiA Testing Construct:'''</font></u> | |||
__NOTOC__ | |||
{| border="1" cellpadding="0" width="100%" | |||
|- | |||
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 | |||
|- | |||
!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart> <br> (PCR'd)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> | |||
|- | |||
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:Plus sign.png|20px]][[Image:B0014 logo.png]]</center> | |||
|- | |||
!style="background:#D9F2ED"|<center><bbpart>J37022</bbpart>=<br>(<bbpart>J04500</bbpart>+<bbpart>J37024</bbpart>)</center>|| || !!style="background:#CCEBAD" | <center>[[Image:J37022 logo.png]]</center> | |||
|} | |||
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page]. | |||
*[ | |||
==<bbpart>J37022</bbpart> | ==Quality control== | ||
__NOTOC__ | |||
{| border="1" cellpadding="0" width="100%" | |||
|- | |||
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing | |||
|- | |||
| <center><bbpart>J37022</bbpart></center> || [[Image:J37022 logo.png]] || No || N/A || N/A | |||
|- | |||
| <center><bbpart>J37023</bbpart></center> || [[Image:J37023 logo.png]] || Yes || [http://openwetware.org/images/b/b3/J37025sequencing.doc Yes] <br> Note: This part was sequenced as a part of J37025 || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used] | |||
|- | |||
| <center><bbpart>J37024</bbpart></center> || [[Image:J37024 logo.png]] || No || N/A || N/A | |||
|} | |||
== | ==WetLab work entries== | ||
*The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information] | |||
*Parts J37024 and J37022 were not built due to time constraints. | |||
* | |||
Latest revision as of 08:06, 30 October 2006
Super Parts | Predator Construct | |||
---|---|---|---|---|
Actual Part | AiiA Testing Construct <bbpart>J37022</bbpart> | |||
Sub Parts | <bbpart>R0010</bbpart> | <bbpart>B0034</bbpart> | <bbpart>J37023</bbpart> | <bbpart>B0015</bbpart> |
Ligation Strategy
Ligating the AiiA Testing Construct:
- More information on ligations can be found on the Ligation Page.
Quality control
Part | Logo | Built | Sequenced | Primers Used for Sequencing |
---|---|---|---|---|
No | N/A | N/A | ||
Yes | Yes Note: This part was sequenced as a part of J37025 |
Primers used | ||
No | N/A | N/A |
WetLab work entries
- The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
- Parts J37024 and J37022 were not built due to time constraints.