IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Killing Predator Construct/Implementation

Super Parts	Predator Construct
Actual Part	AiiA Testing Construct <bbpart>J37022</bbpart>
Sub Parts	<bbpart>R0010</bbpart>	<bbpart>B0034</bbpart>	<bbpart>J37023</bbpart>	<bbpart>B0015</bbpart>

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Ligation Strategy

Ligating the AiiA Testing Construct:

For	Stage 1	Stage 2	Stage 3
<bbpart>J37023</bbpart> (PCR'd)
<bbpart>J37024</bbpart>= (<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37022</bbpart>= (<bbpart>J04500</bbpart>+<bbpart>J37024</bbpart>)

• More information on ligations can be found on the Ligation Page.

Quality control

Part	Logo	Built	Sequenced	Primers Used for Sequencing
<bbpart>J37022</bbpart>		No	N/A	N/A
<bbpart>J37023</bbpart>		Yes	Yes Note: This part was sequenced as a part of J37025	Primers used
<bbpart>J37024</bbpart>		No	N/A	N/A

WetLab work entries

• The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
• Parts J37024 and J37022 were not built due to time constraints.