IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Prey Construct/Design: Difference between revisions

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!height="25pt" width="80pt"|Actual Part
!height="25pt" width="80pt"|Actual Part
!colspan="3"| Logo of the Part
!colspan="3"|[[Image:J37015page.JPG‎|360px]]
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!height="25pt" width="80pt"|Sub Parts
!height="25pt" width="80pt"|Sub Parts
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]  
! [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 F2620]
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]  
! [http://parts.mit.edu/registry/index.php/Part:BBa_C0261 C0261]
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]]  
! [http://parts.mit.edu/registry/index.php/Part:BBa_I13401 I13401]
|}
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Latest revision as of 15:57, 29 October 2006

Super Parts Prey Construct
Actual Part
Sub Parts F2620 C0261 I13401


Registry

J37015

J37015 @ MIT Parts Registry

Design Choices

  • Design allows for an exponential increase of AHL via a positive feedback loop
    • Interaction between LuxR, AHL and promoter controlling expression of LuxI
  • Design allows for assesment of status of positive feedback loop via:
    • GFP Production - Linked to LuxI expression
    • AHL Production - Can be indirectly assessed via T9002 assay


INPUTS biological component comments
AHL LuxR + pLuxR These components sense input and activate output
OUTPUTS biological component comments
AHL LuxI LuxI expression causes enzymatic generation of AHL
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