IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Prey Construct/Modelling: Difference between revisions
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!height="25pt" width="80pt"|Actual Part | !height="25pt" width="80pt"|Actual Part | ||
!colspan="3"| | !colspan="3"|[[Image:J37015page.JPG|360px]] | ||
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!height="25pt" width="80pt"|Sub Parts | !height="25pt" width="80pt"|Sub Parts | ||
! [ | ! [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 F2620] | ||
! [ | ! [http://parts.mit.edu/registry/index.php/Part:BBa_C0261 C0261] | ||
! [ | ! [http://parts.mit.edu/registry/index.php/Part:BBa_I13401 I13401] | ||
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Latest revision as of 15:57, 29 October 2006
| Super Parts | Prey Construct | ||
|---|---|---|---|
| Actual Part | 
| ||
| Sub Parts | F2620 | C0261 | I13401 |
Model assumptions and relevance
- LuxR is at steady state
- A good approximation if LuxR gene is left in an active state while cells are growing in medium
- LuxR and AHL bind in a linear second order fashion
- This is not strictly true as LuxR and AHL bind using pseudo-Michaelis-Menten kinetics
- Gives a good approximation if LuxR is in excess of AHL (ie not saturated by AHL)
- Unlikely to occur all the time as LuxI levels increase to very high amounts
- LuxI can be ignored
- LuxI levels vary throughout, and it is these levels that are directly responsible for AHL production
- This could be a major flaw in the model
Model description
Model variables and parameters
| Variables | ||||
| Name | Description | Initial Value | Confidence | Reference |
|---|---|---|---|---|
| AHL | Starting [AHL], must be >0 | 1 | Unknown | links |
| Parameters | ||||
| Name | Description | Value | Confidence | Reference |
|---|---|---|---|---|
| AHL Degredation | Rate of AHL Degredation | Half-Life = 24 Hours | Known | links |
| AHL Vmax | Maximum rate of production of AHL | 10 | Unknown | links |
| AHL Kd | Value at which [Substrate] gives 50% Vmax | 1000 | Unknown | links |
