IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct: Difference between revisions
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ||
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| | | AHL || AHL-lactonase & fluctuating [AHL] | ||
|} | |} | ||
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[[Image: | [[Image:PredatorAtDeviceLevel.png|thumbnail|right|350px|Predator construct at the device level.]] | ||
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{| border="1" cellpadding="15" width="100%" | |||
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | |||
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| AHL || AHL-lactonase | |||
|} | |} | ||
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[[Image:Predsenspart.png|thumbnail|right|350px|Predator sensing test construct.]] | |||
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ||
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| | | AHL || GFP | ||
|} | |} | ||
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[[Image: | [[Image:Predsensingblackbox.png|thumbnail|right|350px|Predator sensing test construct.]] | ||
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==Expected behavior and performances== | ==Expected behavior and performances== | ||
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! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications | ! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications | ||
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| Input Range(s)|| colspan="2" | | | Input Range(s)|| colspan="2" | [0.01nm,10000nm] | ||
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| colspan="2" | | | Output Range(s)|| colspan="2" | <font color=red>'''?'''</font> | ||
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| Transfer function(s) || [[ | | Transfer function(s) || [[Image:016transfercurve.png|250px]] | ||
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| Robustness || colspan="2" | Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications. | | Robustness || colspan="2" | Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications. |
Latest revision as of 07:21, 29 October 2006
Super Parts | Predator Construct | |||
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Actual Part | ||||
Sub Parts | <bbpart>R0062</bbpart> | <bbpart>B0034</bbpart> | <bbpart>C0062</bbpart> | <bbpart>I13504</bbpart> |
Motivation
- The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
- The Aiia gene has been replaced with GFP to allow direct measurement of protein production
- From this data we can create a transfer function where AHL is the input and GFP the output
- Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
- The part will be used to form the sensing part of the final predator construct
Parts Interface
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