IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 25: | Line 25: | ||
<br style="clear:both"> | <br style="clear:both"> | ||
==Motivation== | ==Motivation== | ||
The purpouse for building and testing this part is so we can | *The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase | ||
*The Aiia gene has been replaced with GFP to allow direct measurement of protein production | |||
From this data we can create a transfer function where AHL is the input and GFP the output | *From this data we can create a transfer function where AHL is the input and GFP the output | ||
*Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator | |||
*The part will be used to form the sensing part of the final predator construct | |||
*The part will form the | |||
==Parts Interface== | ==Parts Interface== | ||
{| border="0" width="100% align="center" | {| border="0" width="100% align="center" | ||
Line 48: | Line 42: | ||
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ||
|- | |- | ||
| AHL || GFP | | <center>AHL<br>prey molecule</center> || <center>GFP</center> | ||
|} | |} | ||
|| | || | ||
[[]] | [[Image:IGEM IMPERIAL PreadatorConstructSystemIcon.png|thumbnail|right|350px|Predator construct at the system level.]] | ||
|} | |} | ||
{| border="0" width="100% align="center" | |||
|- | |||
| | |||
{| border="1" cellpadding="15" width="100%" | |||
|- | |||
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | |||
|- | |||
| <center>AHL<br>prey molecule</center> || <center>GFP</center> | |||
|} | |||
|| | |||
[[Image:PredatorAtDeviceLevel.png|thumbnail|right|350px|Predator construct at the device level.]] | |||
|} | |||
Revision as of 06:34, 29 October 2006
Super Parts | Predator Construct | |||
---|---|---|---|---|
Actual Part | ||||
Sub Parts | <bbpart>R0062</bbpart> | <bbpart>B0034</bbpart> | <bbpart>C0062</bbpart> | <bbpart>I13504</bbpart> |
Motivation
- The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
- The Aiia gene has been replaced with GFP to allow direct measurement of protein production
- From this data we can create a transfer function where AHL is the input and GFP the output
- Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
- The part will be used to form the sensing part of the final predator construct
Parts Interface
|
|