IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation

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(Predator Test Construct)
(Quality control)
Line 55: Line 55:
==Quality control==
==Quality control==
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*These parts were sequenced to determine the correct sequence
 
__NOTOC__
__NOTOC__
{| border="1" cellpadding="0" width="100%"
{| border="1" cellpadding="0" width="100%"
|-
|-
-
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequencing Results !! Primers Used for Sequencing
+
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
|-
|-
-
| <bbpart>BBa_J37016</bbpart> ||jkhkh|| Yes || sequencing analysis and link to sequence results || PSBA2 primers
+
| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|-
|-
-
| <bbpart>BBa_J37020</bbpart> ||kljkljkl|| Yes || sequencing analysis and link to sequence results || PSBA2 primers
+
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
 +
Sequence: TAT TGT CTC ATG AGC GG<br>
 +
Seconday Structure: None
|-
|-
 +
| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
 +
|-
 +
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
 +
|-
 +
| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
 +
|-
 +
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
|}
|}

Revision as of 18:16, 29 October 2006

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Ligating the Polycystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37016</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37016 logo.png

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
Image:J37031 logo.png
<bbpart>J37032</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
Image:R0062 logo.pngImage:I13504 logo.png
<bbpart>J37020</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)
Image:J37020 logo.png

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
Image:J37016 logo.png Yes ? ?
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Forward pSB1(AK3/A3/A2)

Sequence: TAT TGT CTC ATG AGC GG
Seconday Structure: None

<bbpart>J37020</bbpart>
Image:J37020 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes ? ?
<bbpart>J37032</bbpart>
Image:J37032 logo.png Yes ? ?
<bbpart>J37033</bbpart>
Image:J37033 part.png Yes ? ?
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