IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation

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==Ligation strategy==
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<u><font size="2">'''Ligating the Polycystronic Predator 'Sensing' Test Construct:'''</font></u>
<u><font size="2">'''Ligating the Polycystronic Predator 'Sensing' Test Construct:'''</font></u>
__NOTOC__
__NOTOC__
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!style="background:#D9F2ED"|<center><bbpart>J37020</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)</center>|| || || !!style="background:#CCEBAD" | [[Image:J37020 logo.png]]
!style="background:#D9F2ED"|<center><bbpart>J37020</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)</center>|| || || !!style="background:#CCEBAD" | [[Image:J37020 logo.png]]
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*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].
==Quality control==
==Quality control==

Revision as of 22:31, 29 October 2006

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Ligation strategy

Ligating the Polycystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37016</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37016 logo.png

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
Image:J37031 logo.png
<bbpart>J37032</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
Image:R0062 logo.pngImage:I13504 logo.png
<bbpart>J37020</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)
Image:J37020 logo.png
  • More information on ligations can be found on the Ligation Page.

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
Image:J37016 logo.png Yes ? ?
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Forward pSB1(AK3/A3/A2)

Sequence: TAT TGT CTC ATG AGC GG
Seconday Structure: None

<bbpart>J37020</bbpart>
Image:J37020 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes ? ?
<bbpart>J37032</bbpart>
Image:J37032 logo.png Yes ? ?
<bbpart>J37033</bbpart>
Image:J37033 logo.png Yes ? ?

WetLab work entries

  • The polycystronic predator sensing part (J37016) has been successfully built, and tested.
  • The monocystronic predator sensing part (J37020) was not built.
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