IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation

Super Parts	Predator Construct
Actual Part
Sub Parts	<bbpart>R0062</bbpart>	<bbpart>B0034</bbpart>	<bbpart>C0062</bbpart>	<bbpart>I13504</bbpart>

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Ligation strategy

Ligating the Polycystronic Predator 'Sensing' Test Construct:

For	Stage 1	Stage 2	Stage 3
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37016</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For	Stage 1	Stage 2	Stage 3	Stage 4
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37032</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37020</bbpart>= (<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)

• More information on ligations can be found on the Ligation Page.

Quality control

Part	Logo	Built	Sequenced	Primers Used for Sequencing
<bbpart>J37016</bbpart>		Yes	?	?
<bbpart>J37019</bbpart>		Yes	Yes	Forward pSB1(AK3/A3/A2) Sequence: TAT TGT CTC ATG AGC GG Seconday Structure: None
<bbpart>J37020</bbpart>		No	N/A	N/A
<bbpart>J37031</bbpart>		Yes	?	?
<bbpart>J37032</bbpart>		Yes	?	?
<bbpart>J37033</bbpart>		Yes	?	?

WetLab work entries

• The polycystronic predator sensing part (J37016) has been successfully built, and tested.
• The monocystronic predator sensing part (J37020) was not built.