IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation

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Current revision (09:47, 30 October 2006) (view source)
(Quality control)
 
Line 67: Line 67:
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
|-
|-
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| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
+
| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || [http://openwetware.org/images/4/4d/J37016_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers]
|-
|-
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| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
+
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers]
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Sequence: TAT TGT CTC ATG AGC GG<br>
+
-
Seconday Structure: None
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|-
|-
| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
|-
|-
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| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || [http://openwetware.org/images/a/ac/J37020sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers]
|-
|-
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| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
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| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || No || N/A
|-
|-
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| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
+
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || No || N/A
|}
|}

Current revision

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Ligation strategy

Ligating the Polycystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37016</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37016 logo.png

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
Image:J37031 logo.png
<bbpart>J37032</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
Image:R0062 logo.pngImage:I13504 logo.png
<bbpart>J37020</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)
Image:J37020 logo.png
  • More information on ligations can be found on the Ligation Page.

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
Image:J37016 logo.png Yes Yes Primers
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Primers
<bbpart>J37020</bbpart>
Image:J37020 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes Yes Primers
<bbpart>J37032</bbpart>
Image:J37032 logo.png Yes No N/A
<bbpart>J37033</bbpart>
Image:J37033 logo.png Yes No N/A

WetLab work entries

  • The polycystronic predator sensing part (J37016) has been successfully built, and tested.
  • The monocystronic predator sensing part (J37020) was not built.
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