IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation

Super Parts	Predator Construct
Actual Part
Sub Parts	<bbpart>R0062</bbpart>	<bbpart>B0034</bbpart>	<bbpart>C0062</bbpart>	<bbpart>I13504</bbpart>

Ligating the Polycystronic Predator 'Sensing' Test Construct:

For	Stage 1	Stage 2	Stage 3
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37016</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For	Stage 1	Stage 2	Stage 3	Stage 4
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37032</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37020</bbpart>= (<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)

Quality control

Part	Logo	Built	Sequencing Results	Primers Used for Sequencing
<bbpart>BBa_J37016</bbpart>	jkhkh	Yes	sequencing analysis and link to sequence results	PSBA2 primers
<bbpart>BBa_J37020</bbpart>	kljkljkl	Yes	sequencing analysis and link to sequence results	PSBA2 primers