IGEM:IMPERIAL/2006/project/parts

From OpenWetWare

< IGEM:IMPERIAL | 2006 | project(Difference between revisions)
Jump to: navigation, search
(Test Constructs)
Current revision (13:10, 28 October 2006) (view source)
 
(36 intermediate revisions not shown.)
Line 1: Line 1:
-
==Parts created for the oscillator==
+
'''[[IGEM:IMPERIAL/2006/project/parts/Sandbox|Sandbox]]'''
-
*[[IGEM:IMPERIAL/2006/project/Oscillator/Parts|Oscillator Parts]]
+
==Test Constructs==
==Test Constructs==
-
*[[IGEM:IMPERIAL/2006/project/parts/BBa_T9002|'''T9002 - Prey Cell Transfer Function Characterisation''']]
+
*<bbpart>BBa_J37027</bbpart> '''Cre Lox''' - Excision characterisation
 +
**[[IGEM:IMPERIAL/2006/project/parts/creloxprotocal|Cre Lox Protocol]]
 +
 
 +
*<bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation
**[[IGEM:IMPERIAL/Protocols/T9002|T9002 Protocol]]
**[[IGEM:IMPERIAL/Protocols/T9002|T9002 Protocol]]
-
**[[IGEM:Imperial/Results/T9002|T9002 Experimental Data]]
+
**[[IGEM:IMPERIAL/2006/Results/T9002|T9002 Experimental Data]]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
Line 12: Line 14:
**[[IGEM:IMPERIAL/Protocols/T9002_AHL_assay|AHL assay Protocol]]  <font color=red>'''To be finalized'''</font>  
**[[IGEM:IMPERIAL/Protocols/T9002_AHL_assay|AHL assay Protocol]]  <font color=red>'''To be finalized'''</font>  
-
*[[IGEM:IMPERIAL/2006/project/parts/BBa_J37015|'''J37015 - Prey Cell Positive Feedback Loop Characterisation''']]
+
*<bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation
-
**[[IGEM:IMPERIAL/Protocols/J37015|J37015 Protocol]] <font color=red>'''To be finalized'''</font>  
+
**[[IGEM:IMPERIAL/2006/Protocols/J37015|J37015 Protocol]]
 +
**[[IGEM:IMPERIAL/2006/Protocols/J37015sevenhour|New]] <font color=red>'''Re-done to use the chemostat'''</font>
**[[IGEM:Imperial/Results/J37015|J37015 Experimental Data]]
**[[IGEM:Imperial/Results/J37015|J37015 Experimental Data]]
-
*[[IGEM:IMPERIAL/2006/project/parts/BBa_J37016|'''J37016 - Predator Cell Transfer Function Characterisation''']]
+
*<bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation
-
**[[IGEM:IMPERIAL/Protocols/T9002|J37016 Protocol]]
+
**[[IGEM:Imperial/Results/J37016|J37016 Experimental Data]]
**[[IGEM:Imperial/Results/J37016|J37016 Experimental Data]]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
-
*[[IGEM:IMPERIAL/2006/project/parts/BBa_J37022|'''J37022 - Predator Cell AiiA Characterisation''']]
+
*<bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation  
-
**[[IGEM:IMPERIAL/Protocols/J37022|J37022 Protocol]] <font color=green>'''To be cross validated'''</font> 
+
**[[IGEM:Imperial/Results/J37022|J37022 Experimental Data]]
**[[IGEM:Imperial/Results/J37022|J37022 Experimental Data]]
-
*[http://parts.mit.edu/registry/index.php?title=Part:BBa_I13208 '''I13208 - AiiA Zinc Hypothesis Test]
+
*<bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed
-
**[[IGEM:IMPERIAL/Protocols/I13208|I13208 Protocol]] <font color=red> '''Unfinished!!!'''</font>
+
**[[IGEM:Imperial/Results/I13208|I13208 Experimental Data]]
**[[IGEM:Imperial/Results/I13208|I13208 Experimental Data]]
-
*[http://parts.mit.edu/registry/index.php?title=Part:BBa_J37020 '''J37020 - Polycistronic Predator Cell Test Construct]
+
*<bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct
-
**[[IGEM:IMPERIAL/Protocols/T9002|J37020 Protocol]]
+
**[[IGEM:Imperial/Results/J37020|J37020 Experimental Data]]
**[[IGEM:Imperial/Results/J37020|J37020 Experimental Data]]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
-
*'''<bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)'''
+
*<bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)
-
**[[IGEM:IMPERIAL/Protocols/S01656|S01656 Protocol]]
+
**[[IGEM:Imperial/Results/S01656|S01656 Experimental Data]]
**[[IGEM:Imperial/Results/S01656|S01656 Experimental Data]]
-
<br>
 
-
*[http://www.microscopyu.com/articles/livecellimaging/fpintro.html Fluorescent Protein Properties]
 
-
 
-
*[[IGEM:IMPERIAL/2006/project/parts/Tell_us_how_your_part_is_amazing|Guidelines to document your part]]
 
==Miscellaneous Testing==
==Miscellaneous Testing==
-
*[[IGEM:Imperial/Results/ABS490_Calibration|Abs490 -> OD600 Calibration]]
+
*[[IGEM:IMPERIAL/2006/Results/ABS490_Calibration|Abs490 OD600 Calibration]]
-
<br>
+
-
*Monitoring cell population in mixed culture. More information can be found here.[[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21]]
+
-
 
+
-
==Ligations==
+
-
 
+
-
[[Image:Ligation_chart_10_aug.JPG|thumb|left|300px|Ligation Chart (Updated: 14/08)]]
+
-
 
+
-
*[http://openwetware.org/images/b/b1/Ligation_Chart14aug_2006.ppt Ligation Chart]
+
-
*[[IGEM:IMPERIAL/2006/project/parts/lab_status|Lab Status]]
+
*[[IGEM:Imperial/Results/Growth_Media|Testing OD on diferent growth Media]]
-
*[[IGEM:IMPERIAL/2006/project/parts/Primers|Current Primers]]
+
*Monitoring cell population in mixed culture. More information can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21 here]

Current revision

Sandbox

Test Constructs

  • <bbpart>BBa_J37027</bbpart> Cre Lox - Excision characterisation


Miscellaneous Testing

  • Monitoring cell population in mixed culture. More information can be found here
Personal tools