IGEM:IMPERIAL/2006/project/parts
From OpenWetWare
< IGEM:IMPERIAL | 2006 | project(Difference between revisions)
(→Test Constructs) |
Current revision (13:10, 28 October 2006) (view source) |
||
| (12 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
'''[[IGEM:IMPERIAL/2006/project/parts/Sandbox|Sandbox]]''' | '''[[IGEM:IMPERIAL/2006/project/parts/Sandbox|Sandbox]]''' | ||
| - | == | + | ==Test Constructs== |
| - | '''[[IGEM:IMPERIAL/2006/project/parts/ | + | *<bbpart>BBa_J37027</bbpart> '''Cre Lox''' - Excision characterisation |
| - | + | **[[IGEM:IMPERIAL/2006/project/parts/creloxprotocal|Cre Lox Protocol]] | |
| - | + | ||
*<bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation | *<bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation | ||
| Line 16: | Line 15: | ||
*<bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation | *<bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation | ||
| - | **[[IGEM:IMPERIAL/Protocols/J37015|J37015 Protocol]] | + | **[[IGEM:IMPERIAL/2006/Protocols/J37015|J37015 Protocol]] |
| - | **[[IGEM:IMPERIAL/Protocols/J37015sevenhour|New | + | **[[IGEM:IMPERIAL/2006/Protocols/J37015sevenhour|New]] <font color=red>'''Re-done to use the chemostat'''</font> |
**[[IGEM:Imperial/Results/J37015|J37015 Experimental Data]] | **[[IGEM:Imperial/Results/J37015|J37015 Experimental Data]] | ||
*<bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation | *<bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation | ||
| - | |||
**[[IGEM:Imperial/Results/J37016|J37016 Experimental Data]] | **[[IGEM:Imperial/Results/J37016|J37016 Experimental Data]] | ||
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet] | **[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet] | ||
*<bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation | *<bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation | ||
| - | |||
**[[IGEM:Imperial/Results/J37022|J37022 Experimental Data]] | **[[IGEM:Imperial/Results/J37022|J37022 Experimental Data]] | ||
*<bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed | *<bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed | ||
| - | |||
**[[IGEM:Imperial/Results/I13208|I13208 Experimental Data]] | **[[IGEM:Imperial/Results/I13208|I13208 Experimental Data]] | ||
*<bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct | *<bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct | ||
| - | |||
**[[IGEM:Imperial/Results/J37020|J37020 Experimental Data]] | **[[IGEM:Imperial/Results/J37020|J37020 Experimental Data]] | ||
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet] | **[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet] | ||
*<bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG) | *<bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG) | ||
| - | |||
**[[IGEM:Imperial/Results/S01656|S01656 Experimental Data]] | **[[IGEM:Imperial/Results/S01656|S01656 Experimental Data]] | ||
| - | + | ||
==Miscellaneous Testing== | ==Miscellaneous Testing== | ||
| - | *[[IGEM: | + | *[[IGEM:IMPERIAL/2006/Results/ABS490_Calibration|Abs490 OD600 Calibration]] |
*[[IGEM:Imperial/Results/Growth_Media|Testing OD on diferent growth Media]] | *[[IGEM:Imperial/Results/Growth_Media|Testing OD on diferent growth Media]] | ||
*Monitoring cell population in mixed culture. More information can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21 here] | *Monitoring cell population in mixed culture. More information can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21 here] | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
Current revision
Test Constructs
- <bbpart>BBa_J37027</bbpart> Cre Lox - Excision characterisation
- <bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation
- AHL assay based on T9002
- AHL assay Protocol To be finalized
- <bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation
- J37015 Protocol
- New Re-done to use the chemostat
- J37015 Experimental Data
- <bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation
- <bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation
- <bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed
- <bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct
- <bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)
Miscellaneous Testing
- Monitoring cell population in mixed culture. More information can be found here
