IGEM:IMPERIAL/2006/project/parts: Difference between revisions

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*<bbpart>BBa_J37027</bbpart> '''Cre Lox''' - Excision characterisation
*<bbpart>BBa_J37027</bbpart> '''Cre Lox''' - Excision characterisation
**[[IGEM:IMPERIAL/2006/project/parts/creloxprotocal|Cre Lox Protocol]]
**[[IGEM:IMPERIAL/2006/project/parts/creloxprotocal|Cre Lox Protocol]]
**[[IGEM:IMPERIAL/2006/project/parts/creloxdata|Cre lox Data]]


*<bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation
*<bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation
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*<bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation
*<bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation
**[[IGEM:IMPERIAL/2006/Protocols/J37015|J37015 Protocol]]
**[[IGEM:IMPERIAL/2006/Protocols/J37015|J37015 Protocol]]
**[[IGEM:IMPERIAL/Protocols/J37015sevenhour|New Protocol]] <font color=red>'''Re-done to use the chemostat'''</font>
**[[IGEM:IMPERIAL/2006/Protocols/J37015sevenhour|New]] <font color=red>'''Re-done to use the chemostat'''</font>
**[[IGEM:Imperial/Results/J37015|J37015 Experimental Data]]
**[[IGEM:Imperial/Results/J37015|J37015 Experimental Data]]


*<bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation
*<bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation
**[[IGEM:IMPERIAL/Protocols/T9002|J37016 Protocol]]
**[[IGEM:Imperial/Results/J37016|J37016 Experimental Data]]
**[[IGEM:Imperial/Results/J37016|J37016 Experimental Data]]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]


*<bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation  
*<bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation  
**[[IGEM:IMPERIAL/Protocols/J37022|J37022 Protocol]] <font color=green>'''To be cross validated'''</font> 
**[[IGEM:Imperial/Results/J37022|J37022 Experimental Data]]
**[[IGEM:Imperial/Results/J37022|J37022 Experimental Data]]


*<bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed
*<bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed
**[[IGEM:IMPERIAL/Protocols/I13208|I13208 Protocol]] <font color=red> '''Unfinished!!!'''</font>
**[[IGEM:Imperial/Results/I13208|I13208 Experimental Data]]
**[[IGEM:Imperial/Results/I13208|I13208 Experimental Data]]


*<bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct
*<bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct
**[[IGEM:IMPERIAL/Protocols/T9002|J37020 Protocol]]
**[[IGEM:Imperial/Results/J37020|J37020 Experimental Data]]
**[[IGEM:Imperial/Results/J37020|J37020 Experimental Data]]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]
**[http://openwetware.org/images/a/a2/T9002_Data_Spreadsheet.xls Transfer Function Standard Data Spreadsheet]


*<bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)
*<bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)
**[[IGEM:IMPERIAL/Protocols/S01656|S01656 Protocol]]
**[[IGEM:Imperial/Results/S01656|S01656 Experimental Data]]
**[[IGEM:Imperial/Results/S01656|S01656 Experimental Data]]
**[[IGEM:IMPERIAL/Protocols/S01656new|New S01656 Protocol]]
 


==Miscellaneous Testing==
==Miscellaneous Testing==


*[[IGEM:Imperial/Results/ABS490_Calibration|Abs490 -> OD600 Calibration]]
*[[IGEM:IMPERIAL/2006/Results/ABS490_Calibration|Abs490 OD600 Calibration]]


*[[IGEM:Imperial/Results/Growth_Media|Testing OD on diferent growth Media]]
*[[IGEM:Imperial/Results/Growth_Media|Testing OD on diferent growth Media]]


*Monitoring cell population in mixed culture. More information can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21 here]
*Monitoring cell population in mixed culture. More information can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/ProjectCalendar/2006-8-21 here]
==Successful Sequencing of Finished Constructs==
*'''[[IGEM:IMPERIAL/2006/project/parts/pending_sequencing|Sequencing results awaiting arival]]
*'''[[IGEM:IMPERIAL/2006/project/parts/aiiasequence|aiiA sequence analysis]]'''
*[http://openwetware.org/images/b/b1/J37015_sequencing.doc Comparison of observed and expected sequences for J37015]
*[http://openwetware.org/images/4/4d/J37016_sequencing.doc Comparison of observed and expected sequences for J37016]
*[http://openwetware.org/images/e/e3/J37019_sequencing.doc Comparison of observed and expected sequences for J37019]
*[http://openwetware.org/images/a/ac/J37020sequencing.doc Comparison of observed and expected sequences for J37020]
*[http://openwetware.org/images/b/b3/J37025sequencing.doc Comparison of observed and expected sequences for J37025 (includes J37023 within sequence)]

Latest revision as of 10:10, 28 October 2006

Sandbox

Test Constructs

  • <bbpart>BBa_J37027</bbpart> Cre Lox - Excision characterisation


Miscellaneous Testing

  • Monitoring cell population in mixed culture. More information can be found here