IGEM:IMPERIAL/2006/project/parts

Sandbox

Sequencing

aiiA sequence analysis

Test Constructs

• <bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation
  • T9002 Protocol
  • T9002 Experimental Data
  • Transfer Function Standard Data Spreadsheet

• AHL assay based on T9002
  • AHL assay Protocol To be finalized

• <bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation
  • New Protocol Re-done to use the chemostat
  • J37015 Protocol To be finalized
  • J37015 Experimental Data

• <bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation
  • J37016 Protocol
  • J37016 Experimental Data
  • Transfer Function Standard Data Spreadsheet

• <bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation
  • J37022 Protocol To be cross validated
  • J37022 Experimental Data

• <bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed
  • I13208 Protocol Unfinished!!!
  • I13208 Experimental Data

• <bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct
  • J37020 Protocol
  • J37020 Experimental Data
  • Transfer Function Standard Data Spreadsheet

• <bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)
  • S01656 Protocol
  • S01656 Experimental Data
  • New S01656 Protocol

Miscellaneous Testing

• Abs490 -> OD600 Calibration

• Testing OD on diferent growth Media

• Monitoring cell population in mixed culture. More information can be found here

Successful Sequencing of Finished Constructs

• Comparison of observed and expected sequences for J37015
• Comparison of observed and expected sequences for J37016
• Comparison of observed and expected sequences for J37019
• [ Comparison of observed and expected sequences for J37020]
• [ Comparison of observed and expected sequences for J37025 (includes J37023 within sequence)]