IGEM:IMPERIAL/2006/project/parts/BBa J37015/Cre-LoxP system: Difference between revisions

Revision as of 05:30, 1 August 2006

Using cre-recombinase as a switch appears to be, in theory, a very efficient and solid method.
The parts are not available in the registry however, which means that we would have to make them.
The loxP sites are around 20 bases in length and so could be ordered fairly easily.
The cre recombinase site is around a kilobase in length. However there is a good chance that we may be able to obtain this from one of the labs, and if not, plasmids containing the part are commercially available for us to order.
The cre recombinase will also need a promoter that we can activate (eg lacI)
If the parts can be obtained, then we estimate that the length of time for implementation should be under a week.
Proposed Implementation of Cre-LoxP system

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-*[http://www3.interscience.wiley.com/cgi-bin/fulltext/70001850/PDFSTART Cre recombinase: The universal reagent for genome tailoring]
+==Cre-LoxP System==
-*[http://nar.oxfordjournals.org/cgi/content/full/30/17/e90 Cre recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein]
 * Using cre-recombinase as a switch appears to be, in theory, a very efficient and solid method.
 *The parts are not available in the registry however, which means that we would have to make them.
@@ Line 8: / Line 9: @@
 *If the parts can be obtained, then we estimate that the length of time for implementation should be under a week.
 *[http://openwetware.org/wiki/Image:Cre_Pops_Blocker.ppt Proposed Implementation of Cre-LoxP system]
+==Further Reading==
+*[http://www3.interscience.wiley.com/cgi-bin/fulltext/70001850/PDFSTART Cre recombinase: The universal reagent for genome tailoring]
+*[http://nar.oxfordjournals.org/cgi/content/full/30/17/e90 Cre recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein]