IGEM:IMPERIAL/2006/project/parts/BBa T9002: Difference between revisions

Revision as of 07:59, 19 July 2006

The motivation to build this part is to allow us to determine how AHL at different levels activate genes, and use this to assay AHL.

This part allows us to investigate three parameters:

How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
How much AHL is present, by referring to the transfer function
How LuxR levels effect gene activation

The part responds to AHL levels, via activation of pLuxR
From this, we can calibrate a AHL assay based on fluorescence
LuxR is under the control of pTet, allowing different levels of LuxR to be achieved

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 ==Specification==
-This part allows us to do three things:
+The motivation to build this part is to allow us to determine how AHL at different levels activate genes, and use this to assay AHL.
-#Characterise the transfer function of pLuxR (ie How well AHL activates genes at a constant LuxR expression)
-#Use this characterisation to assay AHL levels in cells
-#See how the transfer function alters with varying LuxR levels
+This part allows us to investigate three parameters:
+*How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
+*How much AHL is present, by referring to the transfer function
+*How LuxR levels effect gene activation
+==Design==
+===Part Design===
+[[image:T9002.JPG]]
+[http://parts.mit.edu/registry/index.php/Part:BBa_T9002 T9002 - Link to MIT Parts Registry]
+===Inputs/Outputs===
+*Part Input = AHL
+*Part Output = Green Fluorescent Protein
+===Comments===
+*The part responds to AHL levels, via activation of pLuxR
+*From this, we can calibrate a AHL assay based on fluorescence
+*LuxR is under the control of pTet, allowing different levels of LuxR to be achieved