IGEM:IMPERIAL/2006/project/parts/lab status: Difference between revisions

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|J37015RS(6B->RS->12D->24A)
|J37015RS(6B->RS->12D->24A)
|<font color=blue>'''Re-Ligated'''</font>
|<font color=purple>'''Mini-preped'''</font>
|F2620+Riboswitch+(C0261+I13504), miniprep 18/8
|F2620+Riboswitch+(C0261+I13504), maxiprep 21/8
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Revision as of 07:55, 20 August 2006

Lab Status

This page is for us to list the stage that needed parts are at in the lab. If anyone wants a part cultured that is not listed here, please add it to the bottom of the table.

Status Hierarchy

  • Not Cultured
    • Part is still sitting in iGEM DNA Plate
  • Cultured
    • Part has been transformed into E.coli and has been cultured up
  • Mini-preped
    • DNA has been mini-preped and assessed by restriction digest
  • Maxi-preped
    • DNA has been maxi-preped in preperation for a ligation
  • Ligated
    • DNA has been successfully ligated
    • Please indicate the parts ligated together in the 'Part' column of the table
  • Assayed (I ran out of colours, okay? - TomH)
    • The construct has been successfully assayed

Current Part Status

Part Well Plate Status Remarks Test Part Final Part
F2620 6B 1 Maxi-preped J37015 Prey
T9002 19B 2 Mini-preped Testing phase underway Itself Not in final
C0261 24A 1 Maxi-preped To be ligated to I13504 J37015 Prey
R0062 9G 1 Maxi-preped Too small to miniprep J37019 Predator
B0034 3O 1 Maxi-preped To be ligated to C0062 J37022 Predator, AiiA const
C0062 7A 1 Maxi-preped To be ligated to B0034 J37019 Predator
I13504 12D 1 Maxi-preped To be ligated to C0261 and I13033 separately J37015, J37016 Not in final
I13033 2H 1 Maxi-preped To be ligated to I13504, Successful 2nd attempt (mini-prep). J37020 Not in final
J04500 16P 1 Maxi-preped J37022 Not in final, Cre
C0060 5M 1 Maxi-preped J37022 AiiA const
B0015 1I 1 Maxi-preped J37022 AiiA const, Cre
I13207 2P 1 Mini-preped L-Arabinose located, ready for testing Itself Not in final
I13521 18I 1 Maxi-preped
B0021 1M 1 Cultured
S01656 4G 2 (K res) Mini-preped Testing Phase Underway Itself



Ligation Process

Part Status Remarks Test Part Final Part
B0034+C0062 (7A->3O) Maxi-preped Batch 1 J37019 Predator
I13303+I13504 (12D->2H) Troubleshooting Problem with DNA? J37020 Predator
C0261+I13504 (12D->24A) Maxi-preped J37015 Prey
J37023 Awaiting parts AiiA (C0060)w/FLAG & LVA J37022 J37025
J37024 J37023+B0015 J37022 J37025
J37015 (12D->24A->6B) Maxi-preped, Sequenced F2620+(C0261+I13504) Itself Prey
J37016 (12D->7A->3O->9G) Ligated J37019+I13504, maxiprep 18/8 Itself Predator
J37019 (7A->3O->9G) Maxi-preped, Sent for sequencing R0062+(C0062+B0034) J37016,J37020 Predator
J37020 J37019+(I13033+I13504) Itself Predator
J37022 J04500+J37024 Itself Not in final
J37025 B0034+J37024 see J37022 Predator
RS Part (RS->12D->24A) Maxi-preped, sent for sequencing Riboswitch+(C0261+I13504)
J37015RS(6B->RS->12D->24A) Mini-preped F2620+Riboswitch+(C0261+I13504), maxiprep 21/8
J37019 + B0015 Ligated J37020 Not in final
R0062+I13504 Ligated J37020 Not in final

Parts No Longer Needed

Part Well Plate Status Remarks Test Part Final Part
E0040 5H 1 Mini-preped No longer needed
I13272 4F 1 Mini-preped No longer needed
F2621 4N 1 Mini-preped No longer needed
I13273 4H 1 Mini-preped No longer needed
I13208 4J 1 Mini-preped No longer needed
I0460 11I 1 Cultured No longer needed
F1610 1B 2 Mini-preped No longer needed
I0403 5P 1 Mini-preped No longer needed
E0434 20J 1 Mini-preped No longer needed
I14032 17K 2 Cultured No longer needed
C0260 24C 1 Mini-preped No longer needed
C0160 9F 1 Cultured No longer needed
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