IGEM:IMPERIAL/2006/project/parts/lab status: Difference between revisions

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Line 27: Line 27:
|1
|1
|<font color=green>'''Maxi-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|
|Ligated to C0261+I13504
|J37015
|J37015
|Prey
|Prey
Line 34: Line 34:
|19B
|19B
|2
|2
|<font color=purple>'''Mini-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|Testing phase underway
|Testing phase underway
|Itself
|Itself
Line 77: Line 77:
|To be ligated to C0261 and I13033 separately
|To be ligated to C0261 and I13033 separately
|J37015, J37016
|J37015, J37016
|Not in final
|-
|I13033
|2H
|1
|<font color=green>'''Maxi-preped'''</font>
|To be ligated to I13504, Successful 2nd attempt (mini-prep).
|J37020
|Not in final
|Not in final
|-
|-
Line 91: Line 83:
|1
|1
|<font color=green>'''Maxi-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|
|Ligated to J37024
|J37022
|J37022
|Not in final, Cre
|Not in final, Cre
Line 99: Line 91:
|1
|1
|<font color=green>'''Maxi-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|
|PCR'd to add Immunotag -> J37023
|J37022
|J37022
|AiiA const
|AiiA const
Line 107: Line 99:
|1
|1
|<font color=green>'''Maxi-preped'''</font>  
|<font color=green>'''Maxi-preped'''</font>  
|
|In predator test construct
|J37022
|J37022
|AiiA const, Cre
|AiiA const, Cre
Line 123: Line 115:
|1
|1
|<font color=green>'''Maxi-preped'''</font>  
|<font color=green>'''Maxi-preped'''</font>  
|
|PCR being used to attach LoxP site
|
|J37015Cre
|
|Prey
|-
|-
|B0021
|B0021
Line 131: Line 123:
|1
|1
|<font color=green>'''Maxi-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|
|PCR being used to attach LoxP site
|
|J37015Cre
|
|Prey
|-
|-
|S01656
|S01656
|4G
|4G
|2 (K res)
|2  
|<font color=purple>'''Mini-preped'''</font>
|<font color=purple>'''Mini-preped'''</font>
|Testing Phase Underway
|Testing Phase Underway, Kanamycin resistant
|Itself
|Itself
|
|Not in final
|}
|}


Line 152: Line 144:
!<u>Part</u> !! <u>Status</u> !! <u>Remarks</u> !! <u>Test Part</u> !! <u>Final Part</u>
!<u>Part</u> !! <u>Status</u> !! <u>Remarks</u> !! <u>Test Part</u> !! <u>Final Part</u>
|-
|-
|B0034+C0062 (7A->3O)
|J37015 intermediate
|<font color=green>'''Maxi-preped'''</font> Batch 1
|
|J37019
|Predator
|-
|I13303+I13504 (12D->2H)
|<font color=black>Troubleshooting</font>
|Problem with DNA, have decided to abandon
|J37020
|Predator
|-
|C0261+I13504 (12D->24A)
|<font color=green>'''Maxi-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|
|C0261(24A) + I13504(12D)
|J37015
|J37015
|Prey
|Prey
|-
|-
|J37023
|J37015
|<font color=blue>'''Ligated'''</font>
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|AiiA (C0060)w/FLAG & LVA
|F2620(6B) + (J37015 intermediate)
|J37022
|Itself
|J37025
|Prey
|-
|-
|J37024
|RS Intermediate
|<font color=black></font>
|<font color=green>'''Maxi-preped'''</font>  
|J37023+B0015
|Riboswitch + (C0261(24A)+I13504(12D))
|J37022
|J37015RS
|J37025
|Prey
|-
|-
|J37015 (12D->24A->6B)
|J37015RS
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|<font color=green>'''Maxi-preped, sent for sequencing'''</font>
|F2620+(C0261+I13504)  
|F2620(6B) + (RS Intermediate)
|Itself
|Itself
|Prey
|Prey
|-
|-
|J37016 (12D->7A->3O->9G)
|J37033
|<font color=green>'''Maxi-preped, Sent for sequencing'''</font>  
|<font color=green>'''Maxi-preped'''</font> Batch 1
|J37019+I13504
|B0034(30) + C0062(7A)
|J37019
|Predator test construct
|-
|J37019
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|R0062(9G) + J37033
|J37016
|Predator test constructs, Predator
|-
|J37016
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|J37019 + I13504(12D)
|Itself
|Itself
|Predator
|Predator test construct
|-
|J37031
|<font color=green>'''Maxi-preped'''</font>
|J37019 + B0015(1I)
|J37020
|Predator test construct
|-
|-
|J37019 (7A->3O->9G)
|J37032
|<font color=green>'''Maxi-preped, Sent for sequencing'''</font>  
|<font color=green>'''Maxi-preped'''</font>
|R0062+(C0062+B0034)  
|R0062(9G) + I13504 (12D)
|J37016,J37020
|J37020
|Predator
|Predator test construct
|-
|-
|J37020
|J37020
|<font color=blue>'''Ligated'''</font>
|<font color=green>'''Maxi-preped, sent for sequencing'''</font>
|J37031+J37032
|J37031 + J37032
|Itself
|Itself
|Predator
|Predator test construct
|-
|J37023
|<font color=green>'''Maxi-preped, sent for sequencing'''</font>
|AiiA (C0060)w/FLAG & LVA
|J37024
|Predator, aiiA test construct
|-
|J37024
|<font color=green>'''Maxi-preped</font>
|J37023 + B0015(1I)
|J37022, J37025
|Predator, aiiA test construct
|-
|-
|J37022
|J37022
|<font color=black></font>
|<font color=periwinkle> Being ligated</font>
|J04500+J37024
|J04500 (16P) + J37024
|Itself
|Itself
|Not in final
|aiiA test construct
|-
|-
|J37025
|J37025
|<font color=black></font>
|<font color=periwinkle>Being Ligated</font>
|B0034+J37024
|B0034(30) + J37024
|see J37022
|Predator
|Predator
|Predator
|-
|-
|RS Part (RS->12D->24A)
|J37018
|<font color=green>'''Maxi-preped, sent for sequencing'''</font>  
|<font color=indigo>'''???'''</font>
|Riboswitch+(C0261+I13504)
|J37031 + R0062(9G)
|
|??
|
|Predator
|-
|-
|J37015RS(6B->RS->12D->24A)
|12D->2H
|<font color=green>'''Maxi-preped'''</font>
|<font color=black>Troubleshooting</font>  
|F2620+Riboswitch+(C0261+I13504), need to sequence
|Problem with 2H - abandoned
|
|J37020
|
|Predator
|-
|-
|J37031 (J37019 + B0015)
|J37035
|<font color=green>'''Maxi-preped'''</font>  
|<font color=black></font>  
|see [[IGEM:IMPERIAL/2006/Ligation Page|ligation page]]
|
|
|J37020
|Itself
|Not in final
|-
|-
|J37032 (R0062+I13504)
|J37036
|<font color=green>'''Maxi-preped'''</font>
|<font color=black></font>  
|see [[IGEM:IMPERIAL/2006/Ligation Page|ligation page]]
|
|
|J37020
|Itself
|Not in final
|}
|}


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{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
!<u>Part</u> || <u>Well</u> !! <u>Plate</u> !! <u>Status</u> !! <u>Remarks</u> !! <u>Test Part</u> !! <u>Final Part</u>
!<u>Part</u> || <u>Well</u> !! <u>Plate</u> !! <u>Status</u> !! <u>Remarks</u>
|-
|-
|E0040
|E0040
Line 319: Line 329:
|<font color=orange>'''Cultured'''</font>
|<font color=orange>'''Cultured'''</font>
|No longer needed
|No longer needed
|-
|I13033
|2H
|1
|<font color=green>'''Maxi-preped'''</font>
|Broken Spe1 site
|}


|}
 
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Latest revision as of 09:51, 30 October 2006

Lab Status

This page is for us to list the stage that needed parts are at in the lab. If anyone wants a part cultured that is not listed here, please add it to the bottom of the table.

Status Hierarchy

  • Not Cultured
    • Part is still sitting in iGEM DNA Plate
  • Cultured
    • Part has been transformed into E.coli and has been cultured up
  • Mini-preped
    • DNA has been mini-preped and assessed by restriction digest
  • Maxi-preped
    • DNA has been maxi-preped in preperation for a ligation
  • Ligated
    • DNA has been successfully ligated
    • Please indicate the parts ligated together in the 'Part' column of the table
  • Assayed (I ran out of colours, okay? - TomH)
    • The construct has been successfully assayed

Current Part Status

Part Well Plate Status Remarks Test Part Final Part
F2620 6B 1 Maxi-preped Ligated to C0261+I13504 J37015 Prey
T9002 19B 2 Maxi-preped Testing phase underway Itself Not in final
C0261 24A 1 Maxi-preped To be ligated to I13504 J37015 Prey
R0062 9G 1 Maxi-preped Too small to miniprep J37019 Predator
B0034 3O 1 Maxi-preped To be ligated to C0062 J37022 Predator, AiiA const
C0062 7A 1 Maxi-preped To be ligated to B0034 J37019 Predator
I13504 12D 1 Maxi-preped To be ligated to C0261 and I13033 separately J37015, J37016 Not in final
J04500 16P 1 Maxi-preped Ligated to J37024 J37022 Not in final, Cre
C0060 5M 1 Maxi-preped PCR'd to add Immunotag -> J37023 J37022 AiiA const
B0015 1I 1 Maxi-preped In predator test construct J37022 AiiA const, Cre
I13207 2P 1 Mini-preped L-Arabinose located, ready for testing Itself Not in final
I13521 18I 1 Maxi-preped PCR being used to attach LoxP site J37015Cre Prey
B0021 1M 1 Maxi-preped PCR being used to attach LoxP site J37015Cre Prey
S01656 4G 2 Mini-preped Testing Phase Underway, Kanamycin resistant Itself Not in final



Ligation Process

Part Status Remarks Test Part Final Part
J37015 intermediate Maxi-preped C0261(24A) + I13504(12D) J37015 Prey
J37015 Maxi-preped, Sequenced F2620(6B) + (J37015 intermediate) Itself Prey
RS Intermediate Maxi-preped Riboswitch + (C0261(24A)+I13504(12D)) J37015RS Prey
J37015RS Maxi-preped, sent for sequencing F2620(6B) + (RS Intermediate) Itself Prey
J37033 Maxi-preped Batch 1 B0034(30) + C0062(7A) J37019 Predator test construct
J37019 Maxi-preped, Sequenced R0062(9G) + J37033 J37016 Predator test constructs, Predator
J37016 Maxi-preped, Sequenced J37019 + I13504(12D) Itself Predator test construct
J37031 Maxi-preped J37019 + B0015(1I) J37020 Predator test construct
J37032 Maxi-preped R0062(9G) + I13504 (12D) J37020 Predator test construct
J37020 Maxi-preped, sent for sequencing J37031 + J37032 Itself Predator test construct
J37023 Maxi-preped, sent for sequencing AiiA (C0060)w/FLAG & LVA J37024 Predator, aiiA test construct
J37024 Maxi-preped J37023 + B0015(1I) J37022, J37025 Predator, aiiA test construct
J37022 Being ligated J04500 (16P) + J37024 Itself aiiA test construct
J37025 Being Ligated B0034(30) + J37024 Predator Predator
J37018 ??? J37031 + R0062(9G) ?? Predator
12D->2H Troubleshooting Problem with 2H - abandoned J37020 Predator
J37035 see ligation page Itself
J37036 see ligation page Itself

Parts No Longer Needed

Part Well Plate Status Remarks
E0040 5H 1 Mini-preped No longer needed
I13272 4F 1 Mini-preped No longer needed
F2621 4N 1 Mini-preped No longer needed
I13273 4H 1 Mini-preped No longer needed
I13208 4J 1 Mini-preped No longer needed
I0460 11I 1 Cultured No longer needed
F1610 1B 2 Mini-preped No longer needed
I0403 5P 1 Mini-preped No longer needed
E0434 20J 1 Mini-preped No longer needed
I14032 17K 2 Cultured No longer needed
C0260 24C 1 Mini-preped No longer needed
C0160 9F 1 Cultured No longer needed
I13033 2H 1 Maxi-preped Broken Spe1 site


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