IGEM:IMPERIAL/2006/project/parts/lab status: Difference between revisions

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|J37015 intermediate
|J37015 intermediate
|<font color=green>'''Maxi-preped'''</font>
|<font color=green>'''Maxi-preped'''</font>
|C0261(24A)+I13504(12D)
|C0261(24A) + I13504(12D)
|J37015
|J37015
|Prey
|Prey
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|J37015  
|J37015  
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|F2620(6B)+(J37015 intermediate)
|F2620(6B) + (J37015 intermediate)
|Itself
|Itself
|Prey
|Prey
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|RS Intermediate
|RS Intermediate
|<font color=green>'''Maxi-preped'''</font>  
|<font color=green>'''Maxi-preped'''</font>  
|Riboswitch+(C0261(24A)+I13504(12D))
|Riboswitch + (C0261(24A)+I13504(12D))
|J37015RS
|J37015RS
|Prey
|Prey
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|J37015RS
|J37015RS
|<font color=green>'''Maxi-preped, sent for sequencing'''</font>
|<font color=green>'''Maxi-preped, sent for sequencing'''</font>
|F2620(6B)+(RS Intermediate)
|F2620(6B) + (RS Intermediate)
|Itself
|Itself
|Prey
|Prey
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|J37033  
|J37033  
|<font color=green>'''Maxi-preped'''</font> Batch 1
|<font color=green>'''Maxi-preped'''</font> Batch 1
|B0034(30)+ C0062(7A)
|B0034(30) + C0062(7A)
|J37019
|J37019
|Predator
|Predator
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|J37019
|J37019
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|R0062(9G)+(J37033)
|R0062(9G) + J37033  
|J37016,J37020
|J37016,J37020
|Predator
|Predator
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|J37016  
|J37016  
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|<font color=green>'''Maxi-preped, Sequenced'''</font>  
|J37019+I13504(12D)
|J37019 + I13504(12D)
|Itself
|Itself
|Predator
|Predator

Revision as of 04:26, 1 September 2006

Lab Status

This page is for us to list the stage that needed parts are at in the lab. If anyone wants a part cultured that is not listed here, please add it to the bottom of the table.

Status Hierarchy

  • Not Cultured
    • Part is still sitting in iGEM DNA Plate
  • Cultured
    • Part has been transformed into E.coli and has been cultured up
  • Mini-preped
    • DNA has been mini-preped and assessed by restriction digest
  • Maxi-preped
    • DNA has been maxi-preped in preperation for a ligation
  • Ligated
    • DNA has been successfully ligated
    • Please indicate the parts ligated together in the 'Part' column of the table
  • Assayed (I ran out of colours, okay? - TomH)
    • The construct has been successfully assayed

Current Part Status

Part Well Plate Status Remarks Test Part Final Part
F2620 6B 1 Maxi-preped Ligated to C0261+I13504 J37015 Prey
T9002 19B 2 Mini-preped Testing phase underway Itself Not in final
C0261 24A 1 Maxi-preped To be ligated to I13504 J37015 Prey
R0062 9G 1 Maxi-preped Too small to miniprep J37019 Predator
B0034 3O 1 Maxi-preped To be ligated to C0062 J37022 Predator, AiiA const
C0062 7A 1 Maxi-preped To be ligated to B0034 J37019 Predator
I13504 12D 1 Maxi-preped To be ligated to C0261 and I13033 separately J37015, J37016 Not in final
I13033 2H 1 Maxi-preped To be ligated to I13504, Successful 2nd attempt (mini-prep). J37020 Not in final
J04500 16P 1 Maxi-preped Ligated to J37024 J37022 Not in final, Cre
C0060 5M 1 Maxi-preped J37022 AiiA const
B0015 1I 1 Maxi-preped J37022 AiiA const, Cre
I13207 2P 1 Mini-preped L-Arabinose located, ready for testing Itself Not in final
I13521 18I 1 Maxi-preped PCR being used to attach LoxP site J37015Cre Prey
B0021 1M 1 Maxi-preped PCR being used to attach LoxP site J37015Cre Prey
S01656 4G 2 Mini-preped Testing Phase Underway, Kanamycin resistant Itself Not in final



Ligation Process

Part Status Remarks Test Part Final Part
J37015 intermediate Maxi-preped C0261(24A) + I13504(12D) J37015 Prey
J37015 Maxi-preped, Sequenced F2620(6B) + (J37015 intermediate) Itself Prey
RS Intermediate Maxi-preped Riboswitch + (C0261(24A)+I13504(12D)) J37015RS Prey
J37015RS Maxi-preped, sent for sequencing F2620(6B) + (RS Intermediate) Itself Prey
J37033 Maxi-preped Batch 1 B0034(30) + C0062(7A) J37019 Predator
J37019 Maxi-preped, Sequenced R0062(9G) + J37033 J37016,J37020 Predator
J37016 Maxi-preped, Sequenced J37019 + I13504(12D) Itself Predator
I13303+I13504 (12D->2H) Troubleshooting Problem with DNA, have decided to abandon J37020 Predator
J37023 Maxi-preped, sent for sequencing AiiA (C0060)w/FLAG & LVA, mini/maxiprep 24/8 J37022 J37025
J37024 J37023+B0015 J37022 J37025
J37020 Ligated J37031+J37032, maxi/miniprep 24/8 Itself Predator
J37022 J04500+J37024 Itself Not in final
J37025 B0034+J37024 see J37022 Predator
J37031 Maxi-preped J37019 + B0015 J37020 Not in final
J37032 Maxi-preped R0062 + I13504 J37020 Not in final
J37018 ' J37031+R0062 Predator Cell

Parts No Longer Needed

Part Well Plate Status Remarks
E0040 5H 1 Mini-preped No longer needed
I13272 4F 1 Mini-preped No longer needed
F2621 4N 1 Mini-preped No longer needed
I13273 4H 1 Mini-preped No longer needed
I13208 4J 1 Mini-preped No longer needed
I0460 11I 1 Cultured No longer needed
F1610 1B 2 Mini-preped No longer needed
I0403 5P 1 Mini-preped No longer needed
E0434 20J 1 Mini-preped No longer needed
I14032 17K 2 Cultured No longer needed
C0260 24C 1 Mini-preped No longer needed
C0160 9F 1 Cultured No longer needed
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