IGEM:IMPERIAL/2006/project/parts/lab status
From OpenWetWare
Lab Status
This page is for us to list the stage that needed parts are at in the lab. If anyone wants a part cultured that is not listed here, please add it to the bottom of the table.
Status Hierarchy
- Not Cultured
- Part is still sitting in iGEM DNA Plate
- Cultured
- Part has been transformed into E.coli and has been cultured up
- Mini-preped
- DNA has been mini-preped and assessed by restriction digest
- Maxi-preped
- DNA has been maxi-preped in preperation for a ligation
- Ligated
- DNA has been successfully ligated
- Please indicate the parts ligated together in the 'Part' column of the table
- Assayed (I ran out of colours, okay? - TomH)
- The construct has been successfully assayed
- The construct has been successfully assayed
Current Part Status
| Part | Well | Plate | Status | Remarks | Test Part | Final Part |
|---|---|---|---|---|---|---|
| F2620 | 6B | 1 | Maxi-preped | J37015 | Prey | |
| T9002 | 19B | 2 | Mini-preped | Testing phase underway | Itself | Not in final |
| C0261 | 24A | 1 | Maxi-preped | To be ligated to I13504 | J37015 | Prey |
| R0062 | 9G | 1 | Maxi-preped | Too small to miniprep | J37019 | Predator |
| B0034 | 3O | 1 | Maxi-preped | To be ligated to C0062 | J37022 | Predator, AiiA const |
| C0062 | 7A | 1 | Maxi-preped | To be ligated to B0034 | J37019 | Predator |
| I13504 | 12D | 1 | Maxi-preped | To be ligated to C0261 and I13033 separately | J37015, J37016 | Not in final |
| I13033 | 2H | 1 | Maxi-preped | To be ligated to I13504, Successful 2nd attempt (mini-prep). | J37020 | Not in final |
| J04500 | 16P | 1 | Maxi-preped | J37022 | Not in final, Cre | |
| C0060 | 5M | 1 | Maxi-preped | J37022 | AiiA const | |
| B0015 | 1I | 1 | Maxi-preped | J37022 | AiiA const, Cre | |
| I13207 | 2P | 1 | Mini-preped | L-Arabinose located, ready for testing | Itself | Not in final |
| I13521 | 18I | 1 | Maxi-preped | PCR being used to attach LoxP site | J37015Cre | Prey |
| B0021 | 1M | 1 | Maxi-preped | PCR being used to attach LoxP site | J37015Cre | Prey |
| S01656 | 4G | 2 (K res) | Mini-preped | Testing Phase Underway | Itself | Not in final |
Ligation Process
| Part | Status | Remarks | Test Part | Final Part |
|---|---|---|---|---|
| B0034+C0062 (7A->3O) | Maxi-preped Batch 1 | J37019 | Predator | |
| I13303+I13504 (12D->2H) | Troubleshooting | Problem with DNA, have decided to abandon | J37020 | Predator |
| C0261+I13504 (12D->24A) | Maxi-preped | J37015 | Prey | |
| J37023 | Ligated | AiiA (C0060)w/FLAG & LVA, mini/maxiprep 24/8 | J37022 | J37025 |
| J37024 | J37023+B0015 | J37022 | J37025 | |
| J37015 (12D->24A->6B) | Maxi-preped, Sequenced | F2620+(C0261+I13504) | Itself | Prey |
| J37016 (12D->7A->3O->9G) | Maxi-preped, Sequenced | J37019+I13504 | Itself | Predator |
| J37019 (7A->3O->9G) | Maxi-preped, Sequenced | R0062+(C0062+B0034) | J37016,J37020 | Predator |
| J37020 | Ligated | J37031+J37032, maxi/miniprep 24/8 | Itself | Predator |
| J37022 | J04500+J37024 | Itself | Not in final | |
| J37025 | B0034+J37024 | see J37022 | Predator | |
| RS Part (RS->12D->24A) | Maxi-preped, sent for sequencing | Riboswitch+(C0261+I13504) | ||
| J37015RS(6B->RS->12D->24A) | Maxi-preped | F2620+Riboswitch+(C0261+I13504), need to sequence | ||
| J37031 | Maxi-preped | J37019 + B0015 | J37020 | Not in final |
| J37032 | Maxi-preped | R0062 + I13504 | J37020 | Not in final |
| J37018 | ' | J37031+R0062 | Predator Cell |
Parts No Longer Needed
| Part | Well | Plate | Status | Remarks | Test Part | Final Part |
|---|---|---|---|---|---|---|
| E0040 | 5H | 1 | Mini-preped | No longer needed | ||
| I13272 | 4F | 1 | Mini-preped | No longer needed | ||
| F2621 | 4N | 1 | Mini-preped | No longer needed | ||
| I13273 | 4H | 1 | Mini-preped | No longer needed | ||
| I13208 | 4J | 1 | Mini-preped | No longer needed | ||
| I0460 | 11I | 1 | Cultured | No longer needed | ||
| F1610 | 1B | 2 | Mini-preped | No longer needed | ||
| I0403 | 5P | 1 | Mini-preped | No longer needed | ||
| E0434 | 20J | 1 | Mini-preped | No longer needed | ||
| I14032 | 17K | 2 | Cultured | No longer needed | ||
| C0260 | 24C | 1 | Mini-preped | No longer needed | ||
| C0160 | 9F | 1 | Cultured | No longer needed |
