IGEM:IMPERIAL/2006/project/parts/lab status

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Lab Status

This page is for us to list the stage that needed parts are at in the lab. If anyone wants a part cultured that is not listed here, please add it to the bottom of the table.

Status Hierarchy

  • Not Cultured
    • Part is still sitting in iGEM DNA Plate
  • Cultured
    • Part has been transformed into E.coli and has been cultured up
  • Mini-preped
    • DNA has been mini-preped and assessed by restriction digest
  • Maxi-preped
    • DNA has been maxi-preped in preperation for a ligation
  • Ligated
    • DNA has been successfully ligated
    • Please indicate the parts ligated together in the 'Part' column of the table
  • Assayed (I ran out of colours, okay? - TomH)
    • The construct has been successfully assayed

Current Part Status

Part Well Plate Status Remarks Test Part Final Part
F2620 6B 1 Maxi-preped Ligated to C0261+I13504 J37015 Prey
T9002 19B 2 Maxi-preped Testing phase underway Itself Not in final
C0261 24A 1 Maxi-preped To be ligated to I13504 J37015 Prey
R0062 9G 1 Maxi-preped Too small to miniprep J37019 Predator
B0034 3O 1 Maxi-preped To be ligated to C0062 J37022 Predator, AiiA const
C0062 7A 1 Maxi-preped To be ligated to B0034 J37019 Predator
I13504 12D 1 Maxi-preped To be ligated to C0261 and I13033 separately J37015, J37016 Not in final
J04500 16P 1 Maxi-preped Ligated to J37024 J37022 Not in final, Cre
C0060 5M 1 Maxi-preped PCR'd to add Immunotag -> J37023 J37022 AiiA const
B0015 1I 1 Maxi-preped In predator test construct J37022 AiiA const, Cre
I13207 2P 1 Mini-preped L-Arabinose located, ready for testing Itself Not in final
I13521 18I 1 Maxi-preped PCR being used to attach LoxP site J37015Cre Prey
B0021 1M 1 Maxi-preped PCR being used to attach LoxP site J37015Cre Prey
S01656 4G 2 Mini-preped Testing Phase Underway, Kanamycin resistant Itself Not in final



Ligation Process

Part Status Remarks Test Part Final Part
J37015 intermediate Maxi-preped C0261(24A) + I13504(12D) J37015 Prey
J37015 Maxi-preped, Sequenced F2620(6B) + (J37015 intermediate) Itself Prey
RS Intermediate Maxi-preped Riboswitch + (C0261(24A)+I13504(12D)) J37015RS Prey
J37015RS Maxi-preped, sent for sequencing F2620(6B) + (RS Intermediate) Itself Prey
J37033 Maxi-preped Batch 1 B0034(30) + C0062(7A) J37019 Predator test construct
J37019 Maxi-preped, Sequenced R0062(9G) + J37033 J37016 Predator test constructs, Predator
J37016 Maxi-preped, Sequenced J37019 + I13504(12D) Itself Predator test construct
J37031 Maxi-preped J37019 + B0015(1I) J37020 Predator test construct
J37032 Maxi-preped R0062(9G) + I13504 (12D) J37020 Predator test construct
J37020 Maxi-preped, sent for sequencing J37031 + J37032 Itself Predator test construct
J37023 Maxi-preped, sent for sequencing AiiA (C0060)w/FLAG & LVA J37024 Predator, aiiA test construct
J37024 Maxi-preped J37023 + B0015(1I) J37022, J37025 Predator, aiiA test construct
J37022 Being ligated J04500 (16P) + J37024 Itself aiiA test construct
J37025 Being Ligated B0034(30) + J37024 Predator Predator
J37018 ??? J37031 + R0062(9G) ?? Predator
12D->2H Troubleshooting Problem with 2H - abandoned J37020 Predator
J37035 see ligation page Itself
J37036 see ligation page Itself

Parts No Longer Needed

Part Well Plate Status Remarks
E0040 5H 1 Mini-preped No longer needed
I13272 4F 1 Mini-preped No longer needed
F2621 4N 1 Mini-preped No longer needed
I13273 4H 1 Mini-preped No longer needed
I13208 4J 1 Mini-preped No longer needed
I0460 11I 1 Cultured No longer needed
F1610 1B 2 Mini-preped No longer needed
I0403 5P 1 Mini-preped No longer needed
E0434 20J 1 Mini-preped No longer needed
I14032 17K 2 Cultured No longer needed
C0260 24C 1 Mini-preped No longer needed
C0160 9F 1 Cultured No longer needed
I13033 2H 1 Maxi-preped Broken Spe1 site


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