IGEM:IMPERIAL/2006/project/popsblocker/Implementation

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==Ligation strategy==
==Ligation strategy==
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[http://openwetware.org/images/5/5a/Cre_Pops_Blocker.ppt Proposed Implementation of Cre-LoxP system]
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*Apart from ligating into the necessary plasmid at the end, there are no ligation steps involved in making this device. PCR and PCR fusion are being are used because of the unusual design of the device, in so far as both strands have coding regions. The link below explains the method by which we will use PCR to create the device.
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*[http://openwetware.org/images/5/5a/Cre_Pops_Blocker.ppt Proposed Implementation of Cre-LoxP system (ppt)]
==Primers==
==Primers==

Revision as of 14:26, 29 October 2006


Image:Cre.PNG

Ligation strategy

  • Apart from ligating into the necessary plasmid at the end, there are no ligation steps involved in making this device. PCR and PCR fusion are being are used because of the unusual design of the device, in so far as both strands have coding regions. The link below explains the method by which we will use PCR to create the device.

Primers

Design of primers and method of construction

Commercially available Cre plasmid

WetLab work entries

Jonny Wells
Kirsten Jensen

(08/06 - 10/06)

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