IGEM:IMPERIAL/2007/CFS: Difference between revisions
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== Cell Free Systems as a Chassis == | |||
* What is a chassis | |||
* Introduce CFS as in vitro gene expression machinery systems | |||
=== Chassis Comparison Table === | |||
* Characteristics that we talked earlier + stuff from earlier characterization attempt | |||
'''Advantages''' <br> | |||
'''Disadvamtages''' <br> | |||
== Achievements == | |||
'''Cell Free Extracts''' <br> | |||
* Attempted to make S30 ''E. coli'' cell free extracts using French Press. | |||
* Successfully used commercial S30 extracts from Promega for our experiments. | |||
* Successfully made pre-reaction mixture and implemented it in commcercial cell extracts. | |||
'''Vesicles''' <br> | |||
* Successfully implemented the 'Mineral Oil' Method of vesicle formation. | |||
* Successfully implemented empty vesicle formation and vesicles encapsulating GFP. | |||
* Attempting to enclose cell extracts in vesicles to induce gene expression. | |||
* Attempting to find suitable pore proteins to prolong vesicle and expression lifespan | |||
== Progress at a Glance == | |||
Revision as of 06:41, 6 September 2007
Cell Free Systems
Cell Free Systems as a Chassis
- What is a chassis
- Introduce CFS as in vitro gene expression machinery systems
Chassis Comparison Table
- Characteristics that we talked earlier + stuff from earlier characterization attempt
Advantages
Disadvamtages
Achievements
Cell Free Extracts
- Attempted to make S30 E. coli cell free extracts using French Press.
- Successfully used commercial S30 extracts from Promega for our experiments.
- Successfully made pre-reaction mixture and implemented it in commcercial cell extracts.
Vesicles
- Successfully implemented the 'Mineral Oil' Method of vesicle formation.
- Successfully implemented empty vesicle formation and vesicles encapsulating GFP.
- Attempting to enclose cell extracts in vesicles to induce gene expression.
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
