IGEM:IMPERIAL/2007/Calendar/2007-8-2: Difference between revisions

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=== Laboratory===
=== Laboratory===
The following laboratory-related activities were performed today:
The following laboratory-related activities were performed today:
*Preparation of media in the Teachin Lab.
*Preparation of media in the Teaching Lab.
*Completion of list of required materials.
*Completion of list of required laboratory materials.
*Completion of list of required constructs.
*Completion of list of required constructs.


'''Issues/Considerations'''
'''Issues/Considerations'''
*For the DNA constructs, the nature of the vectors is important.
*For the DNA constructs, the nature of the vectors employed by each team needs to be specified.
*A safety report (?) for each project needs to be forwarded to Mr D. Featherbe.
*A safety report (?) for each project is required by Mr D. Featherbe.


===Wiki===
===Wiki===

Revision as of 16:30, 2 August 2007

We are in Week 4 of the iGEM project.


<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Proposals

Task List

Students

Supervisors

Today's Schedule

Name Alex Anthony Ben Cheuk Dirk James Jerry Lucas Maira Peixuan
0900
0930
1000
1030
1100
1130
1200
1230
1300
1330
1400
1430
1500
1530
1600
1630
1700
1730
1800
Evening

Questions

Debriefing

Laboratory

The following laboratory-related activities were performed today:

  • Preparation of media in the Teaching Lab.
  • Completion of list of required laboratory materials.
  • Completion of list of required constructs.

Issues/Considerations

  • For the DNA constructs, the nature of the vectors employed by each team needs to be specified.
  • A safety report (?) for each project is required by Mr D. Featherbe.

Wiki

Today, marked the start of the restructuring of the heavily overloaded team wiki. A refined structure was decided upon; transformation is expected to be completed by the start of the new week.

Projects

Work on the four projects continued:

HRP

The following tasks were completed:

  • Constructs design
  • Methodologies and protocols employed in the project.
  • List of equipment to be used.
  • Possible application in biofilm project.

Cell-Free System

This team completed the following:

  • Protocols on vesicle formation.
  • Shopping lists for Kirsten.

Issues/Considerations

  • Email Christina about more information (...concerning?).

Cell-by-Date

Work was done on:

  • Completing protocols for CBD

Issues/Considerations

  • Investigation should be made of promoters not only in the registry, but also on openwetware.

Biofilm

  • Literature reading: [AHL] & Protocols for biofilm formation
  • Decision regarding choice of two possible reporters, which would be then be critically compared.
  • Team wiki page required restructuring/refinement. Work commenced, specifically with regards to project description.
  • Devise plan on how to compare results from different chassis'.
  • Plan/Start Modelling.
  • Application of HRP.
  • Name for project.
  • Diffusion of AHL in biofilms.

Project Definition

Yesterday's debriefing yielded a considerable amount of confusion regarding the course of the team's projects; particularly the role of HRP. A new project definition was formulated on the basis of much discussion that took place after Wednesday's debriefing.

Modelling

Imperial's Molecular Predation Oscillator of 2006 was reviewed, with particular emphasis placed on the modelling element. A presentation on the modelling cycle followed & techniques employed will be given tomorrow.