IGEM:IMPERIAL/2007/Calendar/2007-8-6

We are in Week 5 of the iGEM project.

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Task List

Students

Wet Lab

• Gene cloning and amplification

Merging Protocols

• Define Standard Unit

Modelling

• Learn more of parameters for individual applications

Learn Vesicles

• Read up more on Materials, Results and Protocols of vesicle formation
• Contact relevant people on vesicle formation

Project Definition and Logo

• Accurate Project definition
• Come up with team logo

Debriefing

Today's briefing yielded the following topics:

Standard Units

It was unanimously agreed that there was a need for standardization between the proposed chassis, and that a standard unit would have to be determined.

• The unit (for rate) proposed is the GFP per ACV (aqueous cell volume) per second.
• The concept needs to be well-documented, and relevant information placed on the wiki.

Biofilm Formation

• Protocols require refinement, particularly wrt the strains of E.coli used in biofilm formation.

Learn Vesicles

• The day was spent scouring the relevant literature.
• Doubts were raised with regard to the protocols involving mineral oil - the proposed means of vesicle formation.

Project Description

• Needs to be ready by Wednesday, 8 August 2007.
• Logo
• A copy needs to be forwarded by Tuesday, 10:00 AM to both professors for approval.

Wiki

There is still significant confusion regarding the purpose of the wiki. The demonstrators suggested it be used more as a planning/organizing tool. Furthermore, the following :

• New menu setup.
• Status page regarding constructs used.

Contact with supervisors/professors

• It was determined that a weekly report be sent to the professors outlining the week's activities and accomplishments.
• It was also suggested that sub-project meetings with the supervisors be scheduled.