IGEM:IMPERIAL/2007/Calendar/2007-9-11: Difference between revisions

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(New page: We are in Week 10 of the iGEM project __NOTOC__ ==Project Calendar== <calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths forma...)
 
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*Looking at equations relating expression in vesicles - to help with data analysis
*Looking at equations relating expression in vesicles - to help with data analysis


===Wet lab===
===Dry lab===
*
*Doing the wiki
**graphic design
 
===Experimental design (wet lab)===
*24 hr experiment on CBD construct - put on hold
**going to sort the methododlogy first
*Design experiments to test affect of evaporation and how to reduce it
**Mineral oil - possibility but affects oxygen supply too
*Today testing:
**if evaporation prevented by layer of oil
**if layer of oil affects the reading of tehe fluorometer
*Test will be done tomorrow at 37&deg;C using either mineral or paraffin oil (depending on results from today's experiment)
**Will

Revision as of 07:06, 12 September 2007

We are in Week 10 of the iGEM project


Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Vesicles

  • Put pTet-GFP into vesicles - need to see what the result is tonight
  • Looking at equations relating expression in vesicles - to help with data analysis

Dry lab

  • Doing the wiki
    • graphic design

Experimental design (wet lab)

  • 24 hr experiment on CBD construct - put on hold
    • going to sort the methododlogy first
  • Design experiments to test affect of evaporation and how to reduce it
    • Mineral oil - possibility but affects oxygen supply too
  • Today testing:
    • if evaporation prevented by layer of oil
    • if layer of oil affects the reading of tehe fluorometer
  • Test will be done tomorrow at 37°C using either mineral or paraffin oil (depending on results from today's experiment)
    • Will