IGEM:IMPERIAL/2007/Calendar/2007-9-11

We are in Week 10 of the iGEM project

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Vesicles

• Put pTet-GFP into vesicles - need to see what the result is tonight
• Looking at equations relating expression in vesicles - to help with data analysis

Dry lab

• Doing the wiki
  • graphic design

Experimental design (wet lab)

• 24 hr experiment on CBD construct - put on hold
  • going to sort the methododlogy first
• Design experiments to test affect of evaporation and how to reduce it
  • Mineral oil - possibility but affects oxygen supply too
• Today testing:
  • if evaporation prevented by layer of oil
  • if layer of oil affects the reading of tehe fluorometer
• Test will be done tomorrow at 37°C using either mineral or paraffin oil (depending on results from today's experiment)
  • Will test for affect on expression of the samples due to oil (blockage of oxygen)
• T7 should be ready by tomorrow

Problems

• Twinkle can't be moved from the teaching lab - need to talk to biochem lab to use theirs
• DsRED express - will be delivered earlier next week (problem with quality control)
• GFP mut-b3 from the lab - needs to be PCRed and cloned with a tag
• LuxR
  • purity < 50%
  • Can't get exact [LuxR]
  • Need LuxR 10 x the amount of DNA for ID construct

CBD

Meeting scheduled with Vincent after tomorrow's debrief

• Refocus CBD application/specification
  • Results so far don't comply with the specification
• Alex - find out what is stopping expression of the pTet construct below 10 and over 45°C
• Might be useful before meeting to get min working temperature
  • not possible as experiments put on hold until methodology improved