IGEM:IMPERIAL/2007/Calendar/2007-9-7: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Maira Tariq (talk | contribs) (New page: We are in Week 9 of the iGEM project __NOTOC__ ==Project Calendar== <calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%...) |
Maira Tariq (talk | contribs) |
||
| Line 13: | Line 13: | ||
==Meeting with Prof Freemont== | ==Meeting with Prof Freemont== | ||
===Experiments=== | ===Experiments=== | ||
====Initial testing:==== | |||
constructs tested in-vivo and in-vitro | |||
*pTet - find out why doesn't work below 10°C and above 45°C | |||
*pT7 - need to reclone (doesn't have the right RBS) | |||
*pLux - doesn't work below 1nM | |||
====Tests for working conditions:==== | |||
*'''ID:''' | |||
**Test on construct 1 carried out with various [AHL] - works for 3,5,7,10,50nM. Reaches steady state within about 3 and a 1/2 hours | |||
**Carrying out purification of LuxR for construct 2 | |||
Revision as of 08:52, 11 September 2007
We are in Week 9 of the iGEM project
Project Calendar
<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Meeting with Prof Freemont
Experiments
Initial testing:
constructs tested in-vivo and in-vitro
- pTet - find out why doesn't work below 10°C and above 45°C
- pT7 - need to reclone (doesn't have the right RBS)
- pLux - doesn't work below 1nM
Tests for working conditions:
- ID:
- Test on construct 1 carried out with various [AHL] - works for 3,5,7,10,50nM. Reaches steady state within about 3 and a 1/2 hours
- Carrying out purification of LuxR for construct 2
