IGEM:IMPERIAL/2007/Cell By Date/Design: Difference between revisions
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Revision as of 06:10, 19 October 2007
Cell by Date
| Property: | Value | Design Solution | System Level |
|---|---|---|---|
| Health Regulations | System Must not be living replicating bacteria | Use a Cell Free System e.g. Promega's S30 Cell Extract | Chassis |
| Lifespan | System must have a shelf life of 7 days | Protease Inhibitor of Cell Extract Should ensure degradation of Visual Reporter is Minimal Proper Packaging should ensure that evaporation of Cell Free system is so low that system can surive for 7 days |
Chassis |
| Inputs | Isothermal Conditions between 0 & 40 C | Exploit Thermal Dependance of rates of expression | Construct |
| Dynamic conditions eg. steps & ramps | " | Construct | |
| Outputs | System should give a visual signal when beef is off |
Couple constituitive promoter to a Fluoresent Protein eg. RFP | Construct |
| Activation Energy | System Needs to have an activation Energy 30 +/- kJ/mol | To be Determined - this is hard to design for | Construct |
| Response Time | System needs to have a response time under 1 hour | To be Determined - this is hard to design for |
How I arrived at the above design Solutions
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