IGEM:IMPERIAL/2007/Cell By Date/Testing
Cell by Date
--Anthony Lazzaro 08:04, 8 October 2007 (CDT) Hey Guys finally pulled the finger out and am writing up cell by date experiments
1.Determining System Capabilities
Having determined that our construct works in cell extract we set out to determine our system's expression level has as a function of temperature and time.
As can be seen in the above plot our results showed that for isothermal conditions the level of fluoresence and hence expression increased linearly with time. When comparing different expression levels at different temperatures we see that our sytem produces more at higher temperatures which was expected.
By linearly approximating the rate of fluoresence we have been to look at whether we can use an arrhenius type relationship to show how the rate of fluoresence increase with temperature. Doing so has shown that an arrhenius type relationship seems to fit and also our system seems to have an activation energy of 1.5kJ/mol
2.Determining Effect of sampling time on system capabilities
3.Determining Effect of DNA concentration on system capabilities
4.Determining effect of packaging on system capabilities
5.Determining System Capabilities in Vivo