IGEM:IMPERIAL/2007/DNA Constructs: Difference between revisions
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|| || || BBa_I719??? || DsRed & pSB1A3 || Planning || DsRed +[http://parts.mit.edu/registry/index.php/Part:BBa_P1010 BBa_P1010] || Biobrick | || || || BBa_I719??? || DsRed & pSB1A3 || Planning || DsRed +[http://parts.mit.edu/registry/index.php/Part:BBa_P1010 BBa_P1010] || Biobrick Submission | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
Revision as of 09:13, 31 August 2007
Constructs Overview
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details.
Cloning Table
Status of biobricks cloned and their applications:
| I | ID | Part | Description | Status | Ligation Target | Use in Constructs |
| 02 | BBa_I13522 | ptet-GFP | XL1, Midi, Mini, BL21 | --- | Basic construct for chassis characterization | |
| 11 | BBa_E7104 | pT7 GFP | XL1, Midi, Mini, BL21 | --- | CBD test construct (pT7) | |
| I | 18 | BBa_T9002 | ptet-luxR-plux-GFP | XL1, Midi, BL21 | --- | Basic construct for chassis characterization |
| 05 | BBa_R0040 | ptet | XL1, Midi, Mini | DsRed | CBD final (ptet), ID AHL sender | |
| BBa_I719??? | pT7 | Pending | DsRed | CBD final construct (pT7) | ||
| 07 | BBa_F2620 | ptet-luxR-plux | XL1, Midi, Mini | DsRed | ID final construct | |
| 14 | BBa_R0051 | pcI | Problematic | DsRed | CBD final construct (pcI) | |
| 21 | BBa_J23039 | ptet-luxI | XL1, Midi | --- | ID sender construct | |
| 24 | BBa_R0062 | plux | XL1, Midi | --- | ID construct 2 | |
| 25 | BBa_J37032 | plux-GFP | XL1, Midi | --- | ID construct 2 | |
| 26 | BBa_S03119 | ptet-luxR | XL1, Midi | --- | --- | |
| 27 | pSB1A3 | Empty pSB1A3 | Waiting Cloning | --- | --- | |
| 28 | BBa_P1010 | pSB1A3 with ccd | Waiting Cloning | --- | --- | |
| 29 | BBa_P1010 | pSB1A2 with ccd | Waiting Cloning | --- | --- | |
| 13 | BBa_B0015 | Double terminator | XL1, Midi, Mini | --- | General part construction | |
| 17 | BBa_I13507 | RFP | XL1, Midi | --- | Not being used anymore | |
| 19 | BBa_B0034 | strong RBS | XL1, Waiting Midi | --- | General part construction | |
| 23 | BBa_I13504 | GFP | XL1, Midi | pcI | General part construction | |
| BBa_I719??? | DsRed | Ordered | ptet, plux, pcI | Reporter system for ID, Hrp | ||
| BBa_I719??? | HrpR-HrpS | Ordered | ??? | Hrp regulators | ||
| BBa_I719??? | pHrpL | Ordered | ??? | Hrp promoter | ||
| BBa_I719??? | HrpV | Ordered | ??? | Hrp inhibitor system | ||
Ligation Table
Status of parts ligated and their applications:
| I | ID | Part | Description | Status | Parents | Application |
| BBa_I719022 | pcI & GFP | Paused | BBa_R0051 + BBa_I13504 | CBD test construct (pcI) | ||
| BBa_I719??? | pT7 & GFP | Proposed | pT7 + BBa_I13504 | CBD test construct (pT7) | ||
| BBa_I719??? | DsRed & pSB1A3 | Planning | DsRed +BBa_P1010 | Biobrick Submission | ||
| BBa_I719??? | pT7 & DsRed | Planning | pT7 + DsRed | CBD final construct (pT7) | ||
| BBa_I719??? | ptet & DsRed | Planning | BBa_R0040 + DsRed | CBD final construct (ptet) | ||
| BBa_I719??? | pcI-DsRed | Paused | BBa_R0051 + DsRed | CBD final construct (pcI) | ||
| BBa_I719??? | ptet-luxR-plux-DsRed | Pending | BBa_F2620 + DsRed | ID detector final construct 1 | ||
| BBa_I719??? | plux-DsRed | Pending | BBa_R0062 + DsRed | ID detector final construct 2 | ||
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3)]
Parts from the Registry
Physical information of biobricks:
| A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(T) | Location | Conc. |
| A | W | G | 02 | BBa_I13522 | GFP under tetracyclin promoter | pSB1A2 | AmpR | High | 937bp | 3016bp | Well 15H, Plate 2 | 110/100 | |
| A | W | G | 05 | BBa_R0040 | tetracyclin promoter | pSB1A2 | AmpR | High | 54bp | 2133bp | Well 7O, Plate 1 | 60 | |
| A | W | G | 07 | BBa_F2620 | lux promoter, with luxR | pSB1A2 | AmpR | High | 1061bp | 3140bp | Well 6B, Plate 1 | 80 | |
| A | X | G | 11 | BBa_E7104 | T7 GFP | pSB1A2 | AmpR | High | 826bp | 2905bp | Well 13F, Plate 2 | 100 | |
| A | W | G | 13 | BBa_B0015 | Double terminator | pSB1AK3 | A+KR | High | 129bp | 3316bp | Well 1B, Plate 2 | 100 | |
| W | X | 14 | BBa_R0051 | λcI promoter | pSB1A2 | AmpR | High | 49bp | 2128bp | Well 9C, Plate 1 | -- | ||
| A | G | 17 | BBa_I13507 | RFP | pSB1A2 | AmpR | High | 861bp | 2940bp | Well 16N, Plate 1 | 80 | ||
| A | W | G | 18 | BBa_T9002 | GFP under lux promoter with LuxR | pSB1A3 | AmpR | High | 1945bp | 4102bp | Well 19B, Plate 2 | 100 | |
| A | W | 19 | BBa_B0034 | RBS | pSB1A2 | AmpR | High | 12bp | 2091bp | Well 3O, Plate 1 | -- | ||
| A | G | 21 | BBa_J23039 | Constitutive luxI | pSB1A2 | AmpR | High | 860bp | 2939bp | Well 11K, Plate 3 | 140 | ||
| A | W | G | 23 | BBa_I13504 | GFP | pSB1A2 | AmpR | High | 875bp | 2954bp | Well 16E, Plate 1 | 120 | |
| A | G | 24 | BBa_R0062 | lux promoter | pSB1A2 | AmpR | High | 55bp | 2134bp | Well 9G, Plate 1 | 90 | ||
| A | G | 25 | BBa_J37032 | GFP under lux promoter | pSB1A2 | AmpR | High | 938bp | 3017bp | Well 8D, Plate 3 | 100 | ||
| A | G | 26 | BBa_S03119 | constitutive LuxR | pSB1A2 | AmpR | High | 998bp | 3077bp | Well 19J, Plate 1 | 60 | ||
| A | W | 27 | pSB1A3 | pSB1A3 (blank) | pSB1A3 | Amp | High | 0bp | 2157bp | Well 23E, Plate 1 | -- | ||
| A | W | 28 | BBa_P1010 | pSB1A3 (ccd) | pSB1A3 | Amp | High | 675bp | 2832bp | Well 20I, Plate 3 | -- | ||
| A | W | 29 | BBa_P1010 | pSB1A2 (ccd) | pSB1A2 | Amp | High | 675bp | 2754bp | Well 20G, Plate 3 | -- | ||
Our Parts
Physical information of our ligated parts:
| A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(P) | Stock |
| BBa_I719022 | cI with GFP | pSB1A2 | AmpR | High | 933bp | 3988bp | ||||||
| BBa_I791021 | luxI coding region under ptet | pSB1A2 | AmpR | High | 860bp | 2939bp | ||||||
| BBa_I791??? | DsRed under ptet | pSB1A2 | AmpR | High | ||||||||
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Concentrations are given in ng/μl
Part Graveyard
General information on parts we do not like...
| A | W | ID | Name | Description | Plasmid | Size | Status | Notes |
| A | W | 01 | BBa_E0040 | GFP | pSB1A2 | 720bp | XL1, Mini | Redundant |
| A | 03 | BBa_J5528 | pBad-GFP | pSB2K3 | 2093bp | failed-XL1 | ara construct abandoned | |
| A | 04 | BBa_J24677 | plux-GFP | pSB1A2 | 1787bp | XL1, Mini | Wrong GFP ligated | |
| A | 06 | BBa_I0500 | pBad | pSB2K3 | 1210bp | XL1, Mini, Midi | ara construct abandoned | |
| A | W | 08 | BBa_F1610 | luxI | pSB1AK3 | 798bp | XL1, Mini, Midi | Substitute part J23039 |
| A | W | 09 | BBa_R0010 | lacI-plac | pSB1A2 | 200bp | XL1, Mini | Lac construct abandoned |
| A | W | 10 | BBa_E1010 | RFP | pSB2K3 | 681bp | XL1, Mini | Substitute part I13507 |
| A | 12 | BBa_S03550 | araC | pSB1AC3 | 958bp | XL1, Mini, Midi | araC construct abandoned | |
| A | 15 | BBa_J07037 | cI-GFP | pSB2K3 | 912bp | XL1, Mini | No RBS present on this part | |
| A | W | 16 | BBa_E0240 | GFP | pSB1A2 | 876bp | XL1, Midi | Redundant |
| 20 | --- | AraC | pSB1AC3 | 1095bp | Planned | araC construct abandoned | ||
Construct Diagrams
The original (old) diagrams page was moved to old construct diagrams page. This page also contains the obsolete parts.
CBD Constructs
ptet GFP
- Availability: Found in registry as BBa_I13522
pT7 GFP
- Availability: Found in resigry as BBa_E7104
ptet DsRed express
- Availability: Requires ligation
- Promoter can be found in registry as: BBa_R0040
- DsRed express is to be ordered from GeneArt
pT7 DsRed express
- Availability: Requires ligation
- Promoter is being PCRed from plasmid derived externally
- DsRed express is to be ordered from GeneArt
ID Constructs
pLux GFP with LuxR
- Availability: Found in registry as BBa_T9002
pLux GFP with LuxR
- Availability: Found in registry as BBa_J37032
<<Two more constructs with DsRed express yet to be included>>
