IGEM:IMPERIAL/2007/DNA Constructs: Difference between revisions
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__NOTOC__ | |||
<!-- Heading --> | |||
{| style="width:800px; height:80px;" cellpadding="0" border="0" | |||
|- | |||
| width="220px" | <html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html> | |||
| width="390px" | <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center> | |||
| width="110px"| <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html> | |||
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{| style="width:800px;" cellpadding="2" cellspacing="0" align="center" | |||
|- style="background:#000000; color:white;" align="center" | |||
| [[IGEM:IMPERIAL/2007|<font face="Arial" size=1 style="color:#ffffff"> '''Home'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=1 style="color:#ffffff"> '''Cell By Date'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=1 style="color:#ffffff"> '''Infector Detector'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Wet Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Wet Lab'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Dry Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Dry Lab'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Resources|<font face="Arial" size=1 style="color:#ffffff">'''Resources'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Meet The Team|<font face="Arial" size=1 style="color:#ffffff"> '''Meet the | |||
Team'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Entertainment|<font face="Arial" size=1 style="color:#ffffff"> '''Entertainment'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Acknowledgments|<font face="Arial" size=1 style="color:#ffffff"> '''Acknowledgments'''</font>]] | |||
|} | |||
<br> | |||
{| cellpadding="0" border="0" align="center" | |||
|width="200px" class="white" valign="top"| | |||
{|class="white" align="left" cellpadding="0" | |||
|- valign="center" | |||
| {{Click || image=Service_course.png | link=IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook|<font face="Arial" size=2 style="color:#000099">'''Lab Notebook'''</font>]]<br /> <font size=1>Daily Record of Wet Lab</font> | |||
|- | |||
| <br> | |||
|- valign="center" | |||
| {{Click || image=Service_protocols.png | link=IGEM:IMPERIAL/2007/Wet Lab/Protocols| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Protocols|<font face="Arial" size=2 style="color:#000099">'''Protocols'''</font>]]<br /> <font size=1>Fabrication & Testing Protocols</font> | |||
|- | |||
| <br> | |||
|- valign="center" | |||
| {{Click || image=Service_notebook.png | link=IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs|<font face="Arial" size=2 style="color:#000099">'''DNA Constructs'''</font>]]<br /> <font size=1>Overview of DNA Constructs</font> | |||
|- | |||
| <br> | |||
|- valign="center" | |||
| {{Click || image=Scientificwriters.png | link=IGEM:IMPERIAL/2007/Wet Lab/Shopping List| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Shopping List|<font face="Arial" size=2 style="color:#000099">'''Shopping List'''</font>]]<br /> <font size=1>Expenditures on Materials</font> | |||
|} | |||
<br clear="all"> | |||
|width="560px" class="white" valign="top" | | |||
==Constructs Overview== | ==Constructs Overview== | ||
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details. | |||
A [[IGEM:IMPERIAL/2007/DNA_Constructs/New|new page]] is currently being developed! | |||
===Cloning Table=== | ===Cloning Table=== | ||
Status of biobricks cloned and their applications: | |||
{| style="width:95%; background:#33CCFF; border:1px solid #00CCFF" align="center" | {| style="width:95%; background:#33CCFF; border:1px solid #00CCFF" align="center" | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 6: | Line 62: | ||
|width=20px|ID | |width=20px|ID | ||
|width=10%|Part | |width=10%|Part | ||
|width= | |width=17%|Description | ||
|width= | |width=18%|Status | ||
|width= | |width=12%|Ligation Target | ||
|width="--"%|Use in Constructs | |width="--"%|Use in Constructs | ||
<!-- | <!-- PROMOTERS WITH GFP --> | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || ptet-GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || Basic construct for chassis characterization | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || pT7 GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF><small>''Maxi''</small></font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || CBD test construct (pT7) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 30 || --- || pT7 (strong GFP) || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font>|| --- || T7 test-construct | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| | || [[IGEM:IMPERIAL/2007/DNA_Constructs/BBa_J24677|I]] || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || ptet-luxR-plux-GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF><small>''Midi''</small></font>, <font color=#FF6633>BL21</font> || --- || Basic construct for chassis characterization | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| | || || || || || || || | ||
<!-- PROMOTERS ONLY --> | |||
|- style="background:#CCFFFF" align="center" | |||
|| || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || DsRedEx || CBD final (ptet), ID AHL sender | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || || BBa_I719??? || pT7 || ''Ready'' || DsRedEx || CBD final construct (pT7) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || ptet-luxR-plux || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || DsRedEx || ID final construct | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || || || || || || | ||
<!-- ID SPECIFIC BRICKS --> | |||
|- style="background:#CCFFFF" align="center" | |||
|| || 21 || [http://parts.mit.edu/registry/index.php/Part:BBa_J23039 BBa_J23039] || ptet-luxI || <font color=#CC0000>XL1</font>, <font color=#0000FF><small>''Midi''</small></font> || --- || ID sender construct | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 24 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0062 BBa_R0062] || plux || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font> || DsRedEx || ID construct 2 | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 25 || [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] || plux-GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || --- || ID construct 2 | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 26 || [http://parts.mit.edu/registry/index.php/Part:BBa_S03119 BBa_S03119] || ptet-luxR || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font> || --- || --- | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || || || || || || | ||
<!-- BRICKS WITH PLASMID VECTORS --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 27 || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || Empty pSB1A3 || '''Problematic''' || --- || --- | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || || || || || || | ||
<!-- BRICKS FOR CONSTRUCTION [NOT USEFUL] --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || --- || ''General part construction'' | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| | || || 17 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || <font color=#CC0000>XL1</font>, <font color=#0000FF><small>''Midi''</small></font> || --- || ''Not being used anymore'' | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || strong RBS || | || || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || strong RBS || <font color=#CC0000>XL1</font> || --- || ''General part construction'' | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 23 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || pT7 || ''General part construction'' | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || | || || || || || || || | ||
<!-- BRICKS BEING ORDERED --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || DsRed || Ordered || ptet, | || || || BBa_I719??? || DsRed Express || Ordered || ptet, plux, pT7 || Reporter system for ID, Hrp | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || HrpR-HrpS | || || || BBa_I719??? || HrpR-HrpS || Ordered || ??? || Hrp regulators | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || pHrpL || Ordered || ??? || Hrp promoter | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || HrpV || Ordered || ??? || Hrp inhibitor system | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 85: | Line 147: | ||
===Ligation Table=== | ===Ligation Table=== | ||
Status of parts ligated and their applications: | |||
{| style="width:95%; background:#00DD33; border:1px solid #00CC33" align="center" | {| style="width:95%; background:#00DD33; border:1px solid #00CC33" align="center" | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
Line 90: | Line 153: | ||
|width=20px|ID | |width=20px|ID | ||
|width=10%|Part | |width=10%|Part | ||
|width= | |width=17%|Description | ||
|width= | |width=18%|Status | ||
|width=22%|Parents | |width=22%|Parents | ||
|width="--"%|Application | |width="--"%|Application | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || | || || || BBa_I719??? || pT7 & GFP || Planned || pT7 + [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || CBD test construct (pT7) | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || | || || || || || || || | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || | || || || BBa_I719??? || DsRedEx & pSB1A3 || ''Paused'' || DsRedEx + [http://parts.mit.edu/registry/index.php/Part:BBa_P1010 BBa_P1010] || Biobrick Submission | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || | || || || || || || || | ||
|| || | |||
|| || || | |||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || pT7 & DsRedEx || Planned || pT7 + DsRedEx || CBD final construct (pT7) | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || ptet & DsRedEx || Planned || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] + DsRedEx || CBD final construct (ptet) | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || ptet-luxR-plux-DsRedEx || Planned || [http://parts.mit.edu/registry/index.php/Part:BBa_T2620 BBa_F2620] + DsRedEx || ID detector final construct 1 | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || plux-DsRedEx || Planned || [http://parts.mit.edu/registry/index.php/Part:BBa_R0062 BBa_R0062] + DsRedEx || ID detector final construct 2 | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
Line 160: | Line 185: | ||
|} | |} | ||
<font size="-2">Legend: I (Information)</font> | <font size="-2">Legend: I (Information), [Status: <font color=#CC0000>XL1</font> - cloned in XL1-Blue; <font color=#CC00FF>''Maxi''</font> - maxiprepped; <font color=#0000FF>''Midi''</font> - midiprepped; <font color=#00CC00>''Mini''</font> - miniprepped; <font color=#FF6633>BL21</font> - cloned in BL21(DE3); <small>status</small> - stocks running low]</font> | ||
===Parts from the Registry=== | ===Parts from the Registry=== | ||
Physical information of biobricks: | |||
{| style="width:95%; background:#FF3399; border:1px solid #FF0099" align="center" | {| style="width:95%; background:#FF3399; border:1px solid #FF0099" align="center" | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
Line 172: | Line 198: | ||
|width=10%| Name | |width=10%| Name | ||
|witdh="--"| Description | |witdh="--"| Description | ||
|width= | |width=9%| Plasmid | ||
|width=55px| Resist | |width=55px| Resist | ||
|width=55px| Copy | |width=55px| Copy | ||
Line 178: | Line 204: | ||
|width=65px| Size(T) | |width=65px| Size(T) | ||
|width=12%| Location | |width=12%| Location | ||
|width=7%| Conc. <br> (ng/ul) | |||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || | || A || <font color=#0000FF>W</font> || G || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2 || 500 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || | || A || W || G || || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 54bp || 2133bp || Well 7O, Plate 1 || 60 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || W || G || || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1061bp || 3140bp || Well 6B, Plate 1 || 80 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || <font color=#990033>X</font> || <font color=#339900>G</font> || || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP|| [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 826bp || 2905bp || Well 13F, Plate 2 || 100 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || | || A || W || G || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2 || 100/70 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || || G || || 17 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 861bp || 2940bp || Well 16N, Plate 1 || 80 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || | || A || <font color=#0000FF>W</font> || G || || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter with LuxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 || 460 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || | || A || W || || || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || RBS || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 12bp || 2091bp || Well 3O, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || || <font color=#339900>G</font> || || 21 || [http://parts.mit.edu/registry/index.php/Part:BBa_J23039 BBa_J23039] || Constitutive luxI || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 860bp || 2939bp || Well 11K, Plate 3 || 140 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || W || G || || 23 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 875bp || 2954bp || Well 16E, Plate 1 || 120/330 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || || G || || 24 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0062 BBa_R0062] || lux promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 55bp || 2134bp || Well 9G, Plate 1 || 90 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || || G || || 25 || [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] || GFP under lux promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 938bp || 3017bp || Well 8D, Plate 3 || 125/330 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || || | || A || || G || || 26 || [http://parts.mit.edu/registry/index.php/Part:BBa_S03119 BBa_S03119] || constitutive LuxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 998bp || 3077bp || Well 19J, Plate 1 || 60 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || W || <font color=#990033>X</font> || || 27 || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || pSB1A3 (blank) || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || Amp || High || 0bp || 2157bp || Well 23E, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| | || || <font color=#00CC00>W</font> || || || 30 || --- || T7 with GFP(strong) || --- || Kan? || ??? || --- || ??? || --- || 150 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| || || || || || || || || || || || || || | |||
|| || || || || || || || || || || || || | |||
|} | |} | ||
===Our Parts=== | ===Our Parts=== | ||
Physical information of our ligated parts: | |||
{| style="width:95%; background:#FF9933; border:1px solid #FF6633" align="center" | {| style="width:95%; background:#FF9933; border:1px solid #FF6633" align="center" | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
Line 251: | Line 272: | ||
|width=12%| Stock | |width=12%| Stock | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || | || || || || || || [http://parts.mit.edu/registry/index.php/Part:BBa_I719022 BBa_I719022] || cI with GFP || pSB1A2 || AmpR || High || 933bp || 3988bp || | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || | || || || || || || BBa_I791021 || luxI coding region under ptet || pSB1A2 || AmpR || High || 860bp || 2939bp || | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || BBa_I791??? || DsRed under | || || || || || || BBa_I791??? || DsRed Express under ptet || pSB1A2 || AmpR || High || || || | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || || || || || || || || | || || || || || || || || || || || || || | ||
|} | |} | ||
<font size="-2">Legend: A (Available), W (Working) [<font color=#00CC00>W</font> - working ''in vivo'', <font color=##0000FF>W</font> - working ''in vitro'', <font color=#990033>X</font> - faulty], G (Checked on gel) [<font color=#339900>G</font> - incorrect size; <font color=#990033>X</font> - does not appear on gel], S (Sequenced)<br>Concentrations are given in ng/μl</font> | |||
===Part Graveyard=== | ===Part Graveyard=== | ||
General information on parts we do not like... | |||
{| style="width:95%; background:#666666; border:1px solid #444444" align="center" | {| style="width:95%; background:#666666; border:1px solid #444444" align="center" | ||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
Line 276: | Line 297: | ||
|width=10%| Plasmid | |width=10%| Plasmid | ||
|width=65px| Size | |width=65px| Size | ||
|width= | |width=15%| Status | ||
|width="--"%| Notes | |width="--"%| Notes | ||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
|| A || || 04 || BBa_J24677 || plux-GFP || pSB1A2 || 1787bp || | || A || W || 01 || BBa_E0040 || GFP || pSB1A2|| 720bp || XL1, Mini || Redundant | ||
|- style="background:#DDDDDD" align="center" | |||
|| A || || 03 || BBa_J5528 || pBad-GFP || pSB2K3 || 2093bp || failed-XL1 || ara construct abandoned | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || || 04 || BBa_J24677 || plux-GFP || pSB1A2 || 1787bp || XL1, Mini || Wrong GFP ligated | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || || 06 || BBa_I0500 || pBad || pSB2K3 || 1210bp || XL1, Mini, Midi || ara construct abandoned | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || W || 08 || BBa_F1610 || luxI || pSB1AK3 || 798bp || XL1, Mini, Midi|| Substitute part J23039 | |||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
|| A || | || A || W || 09 || BBa_R0010 || lacI-plac || pSB1A2 || 200bp || XL1, Mini || Lac construct abandoned | ||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
|| | || A || W || 10 || BBa_E1010 || RFP || pSB2K3 || 681bp || XL1, Mini || Substitute part I13507 | ||
== | |- style="background:#DDDDDD" align="center" | ||
|| A || || 12 || BBa_S03550 || araC || pSB1AC3 || 958bp || XL1, Mini, Midi || araC construct abandoned | |||
|- style="background:#DDDDDD" align="center" | |||
|| || W || 14 || BBa_R0051 || pcI || pSB1A2 || 49bp || Biobrick || pcI construct abandoned | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || || 15 || BBa_J07037 || cI-GFP || pSB2K3 || 912bp || XL1, Mini || No RBS present on this part | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || W || 16 || BBa_E0240 || GFP || pSB1A2 || 876bp || XL1, Midi || Redundant | |||
|- style="background:#DDDDDD" align="center" | |||
|| || W || 20 || --- || AraC || pSB1AC3 || 1095bp || Planned || araC construct abandoned | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || W || 28 || BBa_P1010 || pSB1A3 (ccd) || pSB1A3 || 0bp || failed-XL1 || Strain not available | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || W || 29 || BBa_P1010 || pSB1A2 (ccd) || pSB1A2 || 0bp || --- || Strain not available | |||
|- style="background:#DDDDDD" align="center" | |||
|| || || || || || || || || | |||
|} | |||
==Construct Diagrams== | |||
The original (old) diagrams page was moved to [[IGEM:IMPERIAL/2007/DNA_Constructs/Con_Diagrams_old|old construct diagrams]] page. This page also contains the obsolete parts. | |||
===CBD Constructs=== | |||
'''ptet GFP''' | |||
[[image:ICGEMS_dvc_Ptet_gfp.png|left|thumb|200px|Ptet promoting GFP]] | |||
*Availability: Found in registry as [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] | |||
<br clear="all"> | <br clear="all"> | ||
'''pT7 GFP''' | |||
[[image:ICGEMS_dvc_PT7_gfp.png|left|thumb|200px|PT7 promoting GFP]] | |||
*Availability: Found in resigry as [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] | |||
<br clear="all"> | <br clear="all"> | ||
'''ptet DsRed express''' | |||
[[image:ICGEMS_dvc_Ptet_rfp.PNG|left|thumb|200px|Ptet promoting dsRed]] | |||
*Availability: Requires ligation | |||
*Promoter can be found in registry as: [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] | |||
*DsRed express is to be ordered from GeneArt | |||
<br clear="all"> | <br clear="all"> | ||
'''pT7 DsRed express''' | |||
[[Image:ICGEMS_dvc_PT7_rfp.PNG|left|thumb|200px|pT7 promoting dsRed]] | |||
*Availability: Requires ligation | |||
*Promoter is being PCRed from plasmid derived externally | |||
*DsRed express is to be ordered from GeneArt | |||
<br clear="all"> | <br clear="all"> | ||
==Constructs | ===ID Constructs=== | ||
'''pLux GFP with LuxR''' | |||
[[image:ICGEMS_sys_Ptet_luxR_Plux_gfp.png|left|thumb|400px|Ptet promoting LuxR, Plux promoting GFP]] | |||
*Availability: Found in registry as [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] | |||
<br clear="all"> | |||
'''pLux GFP with LuxR''' | |||
[[image:ICGEMS_sys_Plux_gfp.png|left|thumb|200px|Plux promoting GFP]] | |||
* | *Availability: Found in registry as [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] | ||
<br clear="all"> | |||
''<<Two more constructs with DsRed express yet to be included>>'' | |||
|} | |||
Latest revision as of 03:21, 2 October 2007
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Constructs OverviewHere are several tables listing the bricks which we are cloning, constructing and using, with their relevant details. A new page is currently being developed! Cloning TableStatus of biobricks cloned and their applications:
Ligation TableStatus of parts ligated and their applications:
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low] Parts from the RegistryPhysical information of biobricks:
Our PartsPhysical information of our ligated parts:
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced) Part GraveyardGeneral information on parts we do not like...
Construct DiagramsThe original (old) diagrams page was moved to old construct diagrams page. This page also contains the obsolete parts. CBD Constructsptet GFP
pT7 GFP
ptet DsRed express
pT7 DsRed express
ID ConstructspLux GFP with LuxR
pLux GFP with LuxR
<<Two more constructs with DsRed express yet to be included>> |