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__NOTOC__ | |||
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{| style="width:800px; height:80px;" cellpadding="0" border="0" | |||
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| width="220px" | <html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html> | |||
| width="390px" | <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center> | |||
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| [[IGEM:IMPERIAL/2007|<font face="Arial" size=1 style="color:#ffffff"> '''Home'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=1 style="color:#ffffff"> '''Cell By Date'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=1 style="color:#ffffff"> '''Infector Detector'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Wet Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Wet Lab'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Dry Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Dry Lab'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Resources|<font face="Arial" size=1 style="color:#ffffff">'''Resources'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Meet The Team|<font face="Arial" size=1 style="color:#ffffff"> '''Meet the | |||
Team'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Entertainment|<font face="Arial" size=1 style="color:#ffffff"> '''Entertainment'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Acknowledgments|<font face="Arial" size=1 style="color:#ffffff"> '''Acknowledgments'''</font>]] | |||
|} | |||
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| {{Click || image=Service_course.png | link=IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook|<font face="Arial" size=2 style="color:#000099">'''Lab Notebook'''</font>]]<br /> <font size=1>Daily Record of Wet Lab</font> | |||
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| {{Click || image=Service_protocols.png | link=IGEM:IMPERIAL/2007/Wet Lab/Protocols| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Protocols|<font face="Arial" size=2 style="color:#000099">'''Protocols'''</font>]]<br /> <font size=1>Fabrication & Testing Protocols</font> | |||
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| {{Click || image=Service_notebook.png | link=IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs|<font face="Arial" size=2 style="color:#000099">'''DNA Constructs'''</font>]]<br /> <font size=1>Overview of DNA Constructs</font> | |||
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| {{Click || image=Scientificwriters.png | link=IGEM:IMPERIAL/2007/Wet Lab/Shopping List| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Shopping List|<font face="Arial" size=2 style="color:#000099">'''Shopping List'''</font>]]<br /> <font size=1>Expenditures on Materials</font> | |||
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==Constructs Overview== | ==Constructs Overview== | ||
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details. | Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details. | ||
A [[IGEM:IMPERIAL/2007/DNA_Constructs/New|new page]] is currently being developed! | |||
===Cloning Table=== | ===Cloning Table=== | ||
Line 19: | Line 72: | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || pT7 GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF><small>''Maxi''</small></font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || CBD test construct (pT7) | || || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || pT7 GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF><small>''Maxi''</small></font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || CBD test construct (pT7) | ||
|- style="background:#CCFFFF" align="center" | |||
|| || 30 || --- || pT7 (strong GFP) || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font>|| --- || T7 test-construct | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 27: | Line 83: | ||
<!-- PROMOTERS ONLY --> | <!-- PROMOTERS ONLY --> | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || | || || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || DsRedEx || CBD final (ptet), ID AHL sender | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || || BBa_I719??? || pT7 || ''Ready'' || DsRedEx || CBD final construct (pT7) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || | || || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || ptet-luxR-plux || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || DsRedEx || ID final construct | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 45: | Line 98: | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 24 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0062 BBa_R0062] || plux || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font> || | || || 24 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0062 BBa_R0062] || plux || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font> || DsRedEx || ID construct 2 | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 25 || [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] || plux-GFP || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font> || --- || ID construct 2 | || || 25 || [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] || plux-GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || --- || ID construct 2 | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 57: | Line 110: | ||
<!-- BRICKS WITH PLASMID VECTORS --> | <!-- BRICKS WITH PLASMID VECTORS --> | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 27 || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || Empty pSB1A3 || ''Problematic' | || || 27 || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || Empty pSB1A3 || '''Problematic''' || --- || --- | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 69: | Line 116: | ||
<!-- BRICKS FOR CONSTRUCTION [NOT USEFUL] --> | <!-- BRICKS FOR CONSTRUCTION [NOT USEFUL] --> | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || --- || ''General part construction'' | || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font>, <font color=#00CC00>''Mini''</font> || --- || ''General part construction'' | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
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|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 23 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || GFP || <font color=#CC0000>XL1</font>, <font color=#0000FF>''Midi''</font> || | || || 23 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || pT7 || ''General part construction'' | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 84: | Line 131: | ||
<!-- BRICKS BEING ORDERED --> | <!-- BRICKS BEING ORDERED --> | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || DsRed || Ordered || ptet, plux, | || || || BBa_I719??? || DsRed Express || Ordered || ptet, plux, pT7 || Reporter system for ID, Hrp | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
Line 111: | Line 158: | ||
|width="--"%|Application | |width="--"%|Application | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || pT7 & GFP || Planned || pT7 + [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || CBD test construct (pT7) | |||
|| || || BBa_I719??? || pT7 & GFP || | |||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
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|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || DsRedEx & pSB1A3 || ''Paused'' || DsRedEx + [http://parts.mit.edu/registry/index.php/Part:BBa_P1010 BBa_P1010] || Biobrick Submission | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
Line 126: | Line 170: | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || pT7 & | || || || BBa_I719??? || pT7 & DsRedEx || Planned || pT7 + DsRedEx || CBD final construct (pT7) | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || ptet & DsRedEx || Planned || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] + DsRedEx || CBD final construct (ptet) | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || ptet-luxR-plux- | || || || BBa_I719??? || ptet-luxR-plux-DsRedEx || Planned || [http://parts.mit.edu/registry/index.php/Part:BBa_T2620 BBa_F2620] + DsRedEx || ID detector final construct 1 | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || BBa_I719??? || plux- | || || || BBa_I719??? || plux-DsRedEx || Planned || [http://parts.mit.edu/registry/index.php/Part:BBa_R0062 BBa_R0062] + DsRedEx || ID detector final construct 2 | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
Line 163: | Line 204: | ||
|width=65px| Size(T) | |width=65px| Size(T) | ||
|width=12%| Location | |width=12%| Location | ||
|width=7%| Conc. | |width=7%| Conc. <br> (ng/ul) | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || <font color=#0000FF>W</font> || G || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2 || | || A || <font color=#0000FF>W</font> || G || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2 || 500 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
Line 171: | Line 212: | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || | || A || W || G || || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1061bp || 3140bp || Well 6B, Plate 1 || 80 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
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|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2 || 100 | || A || W || G || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2 || 100/70 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
Line 186: | Line 224: | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter with LuxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 || | || A || <font color=#0000FF>W</font> || G || || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter with LuxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 || 460 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
Line 195: | Line 233: | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 23 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 875bp || 2954bp || Well 16E, Plate 1 || 120 | || A || W || G || || 23 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13504 BBa_I13504] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 875bp || 2954bp || Well 16E, Plate 1 || 120/330 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
Line 201: | Line 239: | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || || G || || 25 || [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] || GFP under lux promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 938bp || 3017bp || Well 8D, Plate 3 || | || A || || G || || 25 || [http://parts.mit.edu/registry/index.php/Part:BBa_J37032 BBa_J37032] || GFP under lux promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 938bp || 3017bp || Well 8D, Plate 3 || 125/330 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
Line 207: | Line 245: | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || | || A || W || <font color=#990033>X</font> || || 27 || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || pSB1A3 (blank) || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || Amp || High || 0bp || 2157bp || Well 23E, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| | || || <font color=#00CC00>W</font> || || || 30 || --- || T7 with GFP(strong) || --- || Kan? || ??? || --- || ??? || --- || 150 | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
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|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || BBa_I791??? || DsRed under ptet || pSB1A2 || AmpR || High || || || | || || || || || || BBa_I791??? || DsRed Express under ptet || pSB1A2 || AmpR || High || || || | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
Line 287: | Line 322: | ||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
|| A || || 12 || BBa_S03550 || araC || pSB1AC3 || 958bp || XL1, Mini, Midi || araC construct abandoned | || A || || 12 || BBa_S03550 || araC || pSB1AC3 || 958bp || XL1, Mini, Midi || araC construct abandoned | ||
|- style="background:#DDDDDD" align="center" | |||
|| || W || 14 || BBa_R0051 || pcI || pSB1A2 || 49bp || Biobrick || pcI construct abandoned | |||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
Line 295: | Line 333: | ||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
|| || | || || W || 20 || --- || AraC || pSB1AC3 || 1095bp || Planned || araC construct abandoned | ||
|- style="background:#DDDDDD" align="center" | |||
|| A || W || 28 || BBa_P1010 || pSB1A3 (ccd) || pSB1A3 || 0bp || failed-XL1 || Strain not available | |||
|- style="background:#DDDDDD" align="center" | |||
|| A || W || 29 || BBa_P1010 || pSB1A2 (ccd) || pSB1A2 || 0bp || --- || Strain not available | |||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
Line 342: | Line 386: | ||
''<<Two more constructs with DsRed express yet to be included>>'' | ''<<Two more constructs with DsRed express yet to be included>>'' | ||
|} |
Latest revision as of 03:21, 2 October 2007
<html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html> | <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html> |
Home | Cell By Date | Infector Detector | Wet Lab | Dry Lab | Resources | Meet the Team | Entertainment | Acknowledgments |
|
Constructs OverviewHere are several tables listing the bricks which we are cloning, constructing and using, with their relevant details. A new page is currently being developed! Cloning TableStatus of biobricks cloned and their applications:
Ligation TableStatus of parts ligated and their applications:
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low] Parts from the RegistryPhysical information of biobricks:
Our PartsPhysical information of our ligated parts:
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced) Part GraveyardGeneral information on parts we do not like...
Construct DiagramsThe original (old) diagrams page was moved to old construct diagrams page. This page also contains the obsolete parts. CBD Constructsptet GFP
pT7 GFP
ptet DsRed express
pT7 DsRed express
ID ConstructspLux GFP with LuxR
pLux GFP with LuxR
<<Two more constructs with DsRed express yet to be included>> |